The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% ge...

The purpose of the present investigation was to determine the feasibility of using lysolipid-based thermal sensitive liposomes (145nm) for high molecular weight molecule delivery. Fluorescein isothiocyanate conjugate-albumin was used as a model drug (MW 66kDa). Thermal sensitive liposomes, which encapsulated the protein were prepared using a passive encapsulation methodology involving freeze-th...

In this study, total glutathione content was determined in human spermatozoa before and after cryopreservation. Total GSH in fresh semen was 4.47±0.46nmol/10(8) cells. Following semen cryopreservation, GSH decreased to 1.62±0.13nmol/10(8) cells, a 64% reduction (p<0.01). This decrease in GSH content was associated with a decrease in sperm progressive motility (68% of reduction, p<0.01). Additio...

Sperm cryopreservation is a method to preserve sperm samples for a long period. However, the fertility of sperm decreases markedly after freezing and thawing in a certain amount of samples. The aim of the present study was to find useful and reliable predictive biomarkers of the capacity to withstand the freeze-thawing process in human ejaculates. Previous researches have shown that enolase1 (E...

Freeze-storage of epididymal sperm is an important technique for the preservation of gametes in animals, including those becoming extinct. We froze canine sperm recovered from the cauda epididymis and investigated the fertility. The qualities of sperm from the cauda epididymis before freezing were: mean sperm motility, 89.4 +/- 1.6 (SE) %; sperm viability, 89.1 +/- 1.1%; and these were signific...

The resistance of concrete structures to the impact cyclic freezing and thawing is one key long-term characteristics, which further determines operation its service life. To date, alternating freeze-thawing cycles under various operating conditions has been little studied related several manufacturing processes: simple vibrated, variotropic centrifuged, improved vibrocentrifuged. purpose this s...

A model was developed to describe the overall dissolution kinetics of semicrystalline PVA gels prepared by freezing and thawing techniques. The dissolution process was described as a three-step mechanism: detachment-, diffusion-, and disentanglement-controlled dissolution. The unfolding of crystalline regions due to detachment and diffusion was calculated. The lamellar thickness of a PVA crysta...