Protective mechanisms of casein-based microcapsules containing mannitol on Lactobacillus acidophilus and Lactococcus lactis ssp. cremoris, changes in their secondary protein structures, and glass transition of the microcapsules were studied after sprayor freeze-drying and after 10 wk of storage in aluminum foil pouches containing different desiccants (NaOH, LiCl, or silica gel) at 25°C. An in s...

Ballistic powder injection is an emerging needle-free technology. Powder particles are accelerated towards the skin and penetrate the superficial layers. Biopharmaceuticals like vaccines can be placed into the epidermal layer of the skin, widely known to be an attractive site for immunization [1]. Beneath avoiding hazards associated with needle-stick injuries using conventional needles and syri...

An autoradiographic method has been developed by which the presence of soluble intracellular materials can be determined for individual cells (1). Our immediate need for the method was to measure the uptake of H3-thymidine into soluble pools during various stages of the cell life cycle, but it should be applicable to the assay of various soluble radioactive materials in a variety of cell types ...

This paper deals with the freeze-drying process and, in particular, with the optimization of the operating conditions of the primary drying stage. When designing a freeze-drying cycle, process control aims at obtaining the values of the operating conditions (temperature of the heating fluid and pressure in the drying chamber) resulting in a product temperature lower than the limit value of the ...

Freeze-drying is a process used to remove water (or another solvent) from a frozen product, thus increasing its shelf-life. It is extensively used in pharmaceuticals manufacturing, to recover the active pharmaceutical ingredient (and the excipients) from an aqueous solution, as well as in some food processes, because of the low operating temperatures that allow preserving product quality. Moreo...

Improved production methods of starter cultures, which constitute the most important element of probiotic preparations, were investigated. The aim of the presented research was to analyse changes in the viability of Lactobacillus. acidophilus and Bifidobacterium bifidum after stabilization (spray drying, liophilization, fluidization drying) and storage in refrigerated conditions for 4 months. T...

The objectives of this study were to use soybean cake as the raw material for the production of isoflavone powder and compare the effects of different carriers as well as drying methods on the powder quality. Results showed that with spray drying, a level of 40% maltodextrin as carrier produced the highest yield (mass) of isoflavone powder, followed by 10% gelatin and 1% sodium alginate. Howeve...

Lyophilized tissues of mice and blood of sheep, infected with either the Kümm, the Welgevonden or the Ball 3 stock of Cowdria ruminantium, remain infective to mice and sheep after storage at 4 degree C for 90 days. Freeze-dried tissues stored at -18 degrees C and -28 degrees C are still infective after 6 months and 2 years, respectively.

Of six candidate strains of Shigella prepared in Brain Heart Infusion broth as freeze-dried vaccine, low survival rates were obtained with two of the most promising strains. Survival rates with these two strains were increased to acceptable levels when the organisms were suspended in a medium consisting of 8.2% sucrose, 0.01 M phosphate, 0.07% monosodium glutamate, and 2.5% human serum albumin....