In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 μg/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 μg/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10.

Bacillus sp. is a Gram-positive bacterium that is commonly found in seawater. In this study, the genome of marine Bacillus sp. strain G3(2015) was sequenced using MiSeq. The fosfomycin resistant gene fosB was identified upon bacterial genome annotation.

Vancomycin-resistant Enterococcus faecium (VREfm) infections are increasing. Current anti-VREfm options (linezolid and daptomycin) suboptimal. Fosfomycin maintains good efficacy against VREfm chloramphenicol is active ≥ 90% of VREfm. We tested + fosfomycin (CAF+FOS) 10 isolated from blood. MICs were 64 to 512 µg/mL for 8 16 chloramphenicol. The combination decreased both MICs, with a synergic e...

Acute and chronic bacterial prostatitis are considered infections which cumbersome to treat, due the limited available selection of effective antibiotics pharmacologically poor distribution in prostatic tissue. Furthermore, emergence novel antimicrobial resistance patterns, such as extended spectrum β‑lactamase (ESBL)‑producing Escherichia coli (E. coli) along with increasing ...

Antibiotic resistance is a major global health problem and continues to increase, so there need forbetter evaluation therapeutic advice for Urinary Tract Infection (UTI) patients. It had isolated E. colifrom clinical isolates that were resistant antibiotics of β-lactam, the first generation cephalosporinand fluoroquinolone. Characterization bacterial sensitivity should be analyzed other antibio...

OBJECTIVES To characterize at the genomic level the evolution of multiresistance during an outbreak of Klebsiella pneumoniae in a burns intensive care unit. The outbreak involved a DHA-1 β-lactamase-producing strain that later acquired carbapenem and fosfomycin resistance, and in one case colistin resistance. METHODS The genomes of two isolates were sequenced and compared with a previously se...

BACKGROUND There is an urgent need for alternative rescue therapies in invasive infections caused by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the clinical efficacy and safety of the combination of fosfomycin and imipenem as rescue therapy for MRSA infective endocarditis and complicated bacteremia. METHODS The trial was conducted between 2001 and 2010 in 3 Spanish hospit...

We studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. Species studied included 3 common species of Enterobacteriaceae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia; methicillin-susceptible and -resistant Staphylo...