Microbial interactions are closely related to human health, and secreted signal molecules from bacteria determine the gene expression of following bacterial cell density molecule density. However, conventional quantitative analysis number requires several days using standard cultivation methods, detection via microbial is difficult since they in extremely small amounts. In this study, we perfor...

Manganese dioxide (MnO2) nanosheets have recently been demonstrated to be particularly attractive for fluorescent sensing and imaging; however, almost all MnO2 nanosheets-based fluorescent assays have been developed with emissive nanoparticles as the probes. In this study, we developed a novel strategy to use organic dyes, instead of emissive nanoparticles, as the probe to construct a platform ...

Orthodontic adhesive is often left on the tooth surface when a multibracket appliance is debonded, and it is difficult to remove because its colour is similar to that of the tooth. If the adhesive changed colour during debonding, residual adhesive could be more easily removed. This in vitro study evaluated the usefulness of adhesive mixed with a small amount of fluorescent dye for clinical orth...

UV light-induced fluorescence is widely used as a key to reveal residual shell colour patterns of Neogene and Palaeogene molluscs. However, only few examples fluorescent are known from Mesozoic marine shells little about the nature in fossils. Here, reveals previously unseen abundance diversity basal pectinid Pleuronectites laevigatus Middle Triassic Muschelkalk Central Europe. In addition vari...

Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary to short sequence elements within the 16S rRNA common to phylogenetically coherent assemblages of microorganisms, were labeled with tetramethylrhodamine and hybridized to suspensions of fixed cells. Flow cytometry was used to resolve individual target and...

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of ...

Immobilization of functional molecules in ordered microarrays has vast importance for many applications sensing and diagnostics. Here, the immobilization small molecule compounds protein microchannel cantilever spotting (?CS) on epoxy-terminated glass surfaces is studied via ring-opening epoxides by thiol, amine, azide, with purpose creating microscale patterns applications. For this, carrying ...

A dopamine-functionalized carbon nanodot (C-dots) ratiometric fluorescence probe for hypochlorite (ClO−) detection is reported. Fluorescent C-dots with maximal emission at 420 nm are synthesized via the hydrothermal synthesis of 3-hydroxyphenylboric acid 160 °C 8 h. After modified dopamine 5 min, obtained exhibit two emissions and 460 nm. intensity gets quenched addition ClO− fluorescent almost...

An equation allowing estimation of the dissociation constant for binding of a non-fluorescent ligand to the enzyme is presented that is based on the competitive replacement of the ligand by its fluorescent analog. We derived an explicit formula for the probe fluorescence intensity, which is suitable for nonlinear least-squares analysis. We used this formula to evaluate the binding of ATP to the...