OBJECTIVE To analyze the accuracy of the diagnosis of two protocols of indirect immunofluorescence assays for canine visceral leishmaniasis. METHODS Dogs from the seroepidemiological survey conducted in an endemic area of the cities of Araçatuba and Andradina, in Northwestern São Paulo state, in 2003, and in a non-endemic area of the metropolitan region of São Paulo, were used to assess two p...

Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signalling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtaine...

Abstract The objective of this paper is to present the sensor platforms for alpha-fetoprotein (AFP), detection which a biomarker Hepatocellular carcinoma. experiments were designed investigate various AFP via surface Plasmon resonance (SPR) technique. In each step immobilization, antiAFP-AFP interaction and enhancing signal platform was used without fluorescent labelling on ultra-thin film chip...