Irradiance is attenuated rapidly in benthic microalgal mats. This makes it difficult to quantify the relevant light field for the benthic community, which is a necessary parameter for accurate primary production estimates. Photosynthesis of microphytobenthos on estuarine mudflats can be measured using variable fluorescence techniques and can be expressed as relative linear photosynthetic electr...

Several labs have measured the spontaneous and stimulated increases in FM 1-43 fluorescence combined with simultaneous capacitance measurements to confirm the method’s viability (1-4). In these tests, the capacitance of single cells was measured using whole-cell patch clamping. By design, the corresponding fluorescence changes were also measured on single cells, either by monitoring changes in ...

Fluorescence imaging represents a non-invasive tool for revealing and understanding spatial heterogeneity in leaf performance caused by external factors, such as abiotic stress. Sun (Rosa meillandina and Chrysanthemum morifolium) and shade (Spathiphyllum wallisii) plants were used to study their tolerance to heat and high illumination. Fluorescence yield, effective PSII quantum yield and non-ph...

backgrounds: to obtain information about clinical isolates of aspergillus flavus group. methods: we examined 55 isolates [45 clinical, 10 reference (6 from culture collections, 4 local reference)] for toxicology, growth rates, and morphological and physiological characteristics. modified czapek agar (cz) and malt extract agar (mea) were used for observing microscopic morphology and measuring fu...

today, lasers are widely used in biology and medicine, and the majority of healthcenters and hospitals utilize modern laser systems for diagnosis and therapy applications.researchers have introduced different medical applications for different lasers used in surgeries and other medical treatments. medical lasers can be categorized in both diagnosis and therapy branches. main difference between ...

the quenching of quinine fluorescence intensity in the presence of some anions in aqueous solution at ambient temperature has been investigated. the quenching is found to be collisional or dynamical in nature. this study reveals the order of two groups of quencher: nai > nabr > nacl > naf and k2cr2o7 > kmno4 >na2so4 > naclo3. increasing anion size in the both groups leads to an  increase in the...

proline dehydrogenase (prodh; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. the goal of this research was gene cloning and characterization of prodh enzyme from pseudomonas fluorescens pf-5 strain. the gene encoding prodh was isolated by means of pcr amplification and cloned in an iptg inducible t...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

Recent years have seen increasing popularity of stochastic chemical kinetic models due to their ability to explain and model several critical biological phenomena. Several developments in high resolution fluorescence microscopy have enabled researchers to obtain protein and mRNA data on the single cell level. The availability of these data along with the knowledge that the system is governed by...