We report on a novel acousto-optic tunable filter (AOTF)-based near-infrared (NIR) fluorescence excitation-emission matrix (EEM) spectroscopy technique for rapid multifluorophore characterization. We implement a unique light filtering module design by using cascaded AOTFs coupled with three orthogonally oriented polarizers to effectively remove the side-ripple artifacts of AOTFs as well as by u...

A low molecular weight protein (approximately 25,000 D) exhibiting a yellow fluorescence emission peaking at approximately 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (lambda max = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the ...

We report electrochemical studies, spectroscopy, and electrogenerated chemiluminescence (ECL) of four monodisperse star-shaped truxene core-oligofluorene compounds (T1-T4). All oligomers produced stable radical anions and radical cations and showed blue ECL by ion annihilation with an intensity that could be seen with the naked eye. ECL spectra showed that all ECL emissions were at the same pos...

The zones can be measured by the fluorescence intensity emitted at an angle of 45'. The height and area of the peaks over the different zones corresponding to the fluorescence emission of that zone may be measured as relative percentages of the total fluorescence. The method uses unconcentrated early morning urine: 20 ILI are applied to paper and electrophoresed for four hours at 200 volts in M...

A series of 6-styryl-2,4-diphenylpyrylium salts exhibiting dual fluorescence has been investigated by fluorescence up-conversion in conjunction with quantum chemical calculations. The short-wavelength emission is due to an excited state localized on the pyrylium fragment and the long-wavelength emission arises from a charge-transfer state delocalized over the whole molecule. The two fluorescing...

A series of four donor aryl alkynyl substituted thiazole derivatives 3a-d and three similar aryl donor-acceptor systems 6a-c have been synthesized. All compounds bear different electron-donating groups in the 5-position of the thiazole core. The influence of both electron donor strength and the additional phenylethynyl unit on photophysical properties, i.e. UV/Vis absorption, fluorescence emiss...

A comprehensive photophysical characterization of a bis-naphthalene derivative, L, was carried out in acetonitrile, using UV-Vis absorption, steady-state and time-resolved emission spectroscopy. It was found that in the 250-400 nm region, the absorption spectra showed two bands with maxima at ~ 220 nm and ~292 nm whereas the emission spectrum showed a band settled between 300 and 550 nm. A deta...