Protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in Saccharomyces cerevisiae and Kluyveromyces marxianus. Crude protein content of dried yeast cells were 53.22% and 45.6% for S.cerevisiae and K.marxianus, respectively. After 96 hrs of...

The purpose of this study was to evaluate the antioxidative activities of Crab meat analogue prepared with protein hydrolysates obtained from mechanically deboned chicken meat (MDCM) from spent laying hens. 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical-scavenging activity was increased by adding MDCM hydrolysates during storage, and activity correlated with the concentration of DPPH adde...

Enzymatic hydrolysis led to improve functional properties and biological activity of protein by-products, which can be further used as protein ingredients for food and feed applications. The effects of proteolytic enzyme modification of egg-yolk protein preparation (YP) and white protein preparation (WP), obtained as the by-products left during the course of lecithin, lysozyme, and cystatin iso...

The degree of hydrolysis and antioxidant activity protein hydrolysates from fresh cow’s milk whey obtained by the action proteolytic enzymes papain, bromelain chymosin were compared. lowest in was reported for sample MP1 (10 min reaction time, treatment with 0.1 mg/ml papain), highest percentage at hydrolysate MB12 (at 60 1.0 bromelain). For samples WC1 μl/ml chymosin), lowest. achieved WP12 us...

Novel antihypercholesterolemic bioactive peptides (BAP) from peptic camel whey protein hydrolysates (CWPH) were generated at different time, temperature, and enzyme concentration (%). Hydrolysates showed higher pancreatic lipase- (PL; except 3 CWPH) cholesterol esterase (CE)-inhibiting potential, as depicted by lower half-maximal inhibitory values (IC50 values) compared with nonhydrolyzed prote...

Lipoxygenases are non-heme iron-containing enzymes that catalyze the oxidation of polyunsaturated fatty acids, resulting in production lipid hydroperoxides, which precursors inflammatory mediators. These widely distributed humans, other eukaryotes, and cyanobacteria. hold promise as therapeutic targets for several human diseases, including cancer inflammation-related disorders. Inhibitors lipox...

There is increasing interest in dietary protein for management of Type 2 diabetes mellitus (T2DM) and obesity. The effects twice-daily oral administration a salmon skin gelatin hydrolysate (SSGH, 50 mg/kg), boarfish (BPH, (50 metformin (200 or saline control, were investigated ob/ob mice. Non-fasting blood glucose was significantly reduced with SSGH (p<0.01), BPH (p<0.001) (p<0.001), w...

Yoghurt strain Lactobacillus LBL-4 cultivated for 8-10 h at pH ~6.0 was investigated as a considerable food-grade source of intracellular aminopeptidase. Cell-free extract manifesting >200 AP U/l was obtained from cells harvested from 1 L culture media. Subtilisin-induced hydrolysates of casein, soybean isolate, and Scenedesmus cell protein with degree of hydrolysis 20-22% incubated at 45°C for...