We have prepared a dry film for the enzymic determination of total serum cholesterol. It consists of a transparent support bearing a buffered gelatin layer, and a white reflective spreading layer that contains all of the necessary components for the detection of cholesterol. The method is based on (a) hydrolysis of cholesterol esters to cholesterol by cholesterol ester hydrolase (EC 3.1.1.13), ...

AIMS To develop a rapid, sensitive, and safe method for the analysis of von Willebrand factor (vWf) multimers in plasma or platelet lysates. METHOD Analysis of vWf multimers was carried out by sodium dodecyl sulphate-agarose discontinuous gel electrophoresis followed by protein transfer to nitrocellulose membranes by western blotting. Blots were probed using horseradish peroxidase (HRP) conju...

Summary In a cross-sectional and follow-up study, we evaluated age-related changes of hand bone mineral density in both sexes using data obtained by digital radiographic densitometry in a large Chuvashian cohort. Objectives The aim of the study was to evaluate age-related changes of hand bone mineral density (BMD) in both sexes using data obtained by digital radiographic densitometry in a large...

Accurate determination of retinal vessel width measurement is important in the study of the haemodynamic changes that accompany various physiological and pathological states. Currently the width at the half height of the transmittance and densitometry profiles are used as a measure of retinal vessel width. A consistent phenomenon of two 'kick points' on the slopes of the transmittance and densi...

AIMS A study was conducted to determine the capability of the recently developed National Eye Institute (NEI) Scheimpflug cataract imaging system in detecting changes in the nuclear region of the lens over a 1 year period. METHODS Twenty five eyes with pure nuclear cataracts with mean nuclear densities < or = 0.30 optical density units (ODU) as well as 30 normal control eyes were each examine...

Objective: This study aimed to investigate the anti-inflammatory activity of methanolic leaf extract Houttuynia cordata Thunb. from Mizoram, India, using in-vitro and in-vivo models. In addition, also determine quercetin content high-performance thin-layer chromatography (HPTLC) densitometry. Methods: The H. was prepared successive Soxhlet extraction, its evaluated models such as inhibition pro...

OBJECTIVE To describe how clinicians deal with the uncertainty inherent in medical evidence in clinical consultations. DESIGN Qualitative study. SETTING Clinical consultations related to hormone replacement therapy, bone densitometry, and breast screening in seven general practices and three secondary care clinics in the UK NHS. PARTICIPANTS Women aged 45-64. RESULTS 45 of the 109 relev...

Eighteen osteoporotic (Z-score at or below 2.5) nonambulatory children with cerebral palsy or a neuromuscular disorder received cyclic intravenous administration of pamidronate. One year after treatment, bone densitometry showed an improvement in all patients: a mean increase of 13% +/- 15% standard deviation (SD) (p < 10(-5)) on the global data, and a mean increase of 27% +/- 15% (p < 10(-5)) ...

A method of making a visual display of subchondral mineralization in the major synovial joints is described. Unlike existing procedures, it can be used on the living subject. A modified application of computed tomography-densitometry, computed tomography-osteoabsorptiometry makes it possible to explore the mechanical adaptability to the prevailing mechanical force. This claim is based upon the ...

Incidental detection of lumbar Paget's disease by bone densitometry SIR, Bone densitometry of the hip and lumbar spine using dual-energy X-ray absorptiometry (DXA) is used to diagnose osteoporosis. We report three patients who were referred for bone densitometry, in whom a diagnosis of Paget's disease was made after the investigation of significantly increased bone mineral density (BMD) of a si...