The flat gel acrylamide electrophoresis technique was standardized and applied to the comparison of four species of porcine mycoplasmas. Clear differences were observed between these species, and differences were seen among the strains of Mycoplasma hyosynoviae. The clarity of the patterns and the number of bands developed was influenced by the amount of protein in the extract and the age of th...

Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifi...

To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and ...