background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

BACKGROUND Kyasanur Forest disease (KFD) is a febrile illness characterized by hemorrhages, and is reported endemic in the Shimoga district in Karnataka state, India. It is caused by the KFD virus (KFDV) of the family Flaviviridae, and is transmitted to monkeys and humans by Haemaphysalis ticks. FINDINGS We investigated a new focus of KFD among tribals in a reserve forest in Kerala state, Ind...

Background: A novel coronavirus led to a rapidly spreading outbreak of COVID-19, which caused morbidity and mortality worldwide. Appropriate case definitions can help diagnose COVID-19. Objectives: This study aimed evaluate the COVID-19 clinical symptoms their potential patterns using latent class analysis (LCA) for identifying confirmed cases among hospitalized patients in northern Iran accord...

SARS-CoV-2 causing corona virus disease 2019 (COVID-19) is the most common infection in recent days worldwide. Rapidly evolving from epidemic outbreak Wuhan, China on 29 December 2019, third known that causes fatal respiratory diseases humans. The roles of biomarkers a blood sample diagnosis COVID-19 patients who have visited hospital with history fever has been evaluated. A retrospective study...

ObjectivesTo our knowledge no previous study has assessed the performance of a rapid antigen diagnostic immunoassay (RAD) conducted at point care (POC). We evaluated Panbio™ COVID-19 Ag Rapid Test Device for diagnosis coronavirus 2019 disease (COVID-19) in symptomatic patients (n = 412) attending primary healthcare centres.MethodsRAD was performed immediately after sampling following manufactur...

canine ehrlichiosis has emerged as one of the most clinically important tick-borne diseases affecting dogs. eighty-five naturally infected dogs have been investigated, the most consistent clinical signs recorded were fever, emaciation and lymphoadenopathy, anemia, monocytosis, thrompocytopenia, hypoalbuminemia, elevation in liver enzymes and total bilirubin were the most remarkable changes asso...