Human stem cells and progenitor cells from the bone marrow have been proposed for the regeneration of ischemic cardiac tissues. Early clinical trials indicate that infusion of autologous bone-marrow cells into the infarcted heart enhances ventricular function, albeit the long-term benefit remains to be ascertained. Alternatively, angiogenic growth factors could be used to stimulate the recruitm...

ex vivo expansion of human umbilical cord blood cells (hucbc) is explored by several investigators to enhance the repopulating potential of hucbc. the proliferation and expansion of human hematopoietic stem cells (hsc) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding hsc for patient transplantation. using primary human mesenchymal stem cells...

The general utility of a novel, fluorescence-based procedure for assessing gene transfer and expression has been demonstrated using hematopoietic stem and progenitor cells. Lineage-depleted hematopoietic cells were isolated from the bone marrow or fetal livers of acid sphingomyelinase-deficient mice, and retrovirally transduced with amphotropic or ecotropic vectors encoding a normal acid sphing...

Cell therapy has been proposed as an alternative for treatment of neurodegenerative disorder. Parkinson’s Diseases (PD) are caused by specific and selective loss of nigrostriatal dopaminergic neurons [1]. The possibility of replacing those cells has been explored through the transplantation of embryonic stem cells (ESCs), fetal stem cells, induced pluripotential stem cells (iPSCs), and mesenchy...

Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. MaterialsAndMethods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (...

During embryonic development, multilineage HSCs/progenitor cells are derived from specialized endothelial cells, termed hemogenic endothelium, within the yolk sac, placenta, and aorta. Whether hemogenic endothelial cells contribute to blood cell development at other sites of definitive hematopoiesis, such as in the fetal liver and fetal bone marrow, is not known. Also unknown is whether such ce...

background: in most cancer cases, the treatment choice for a pregnant patient is radiotherapy. in these patients, the abdomen is usually not exposed therefore fetus exposure is due to peripheral dose (pd). the purpose of this study was to estimate the fetal dose (the maximum pd in each pregnancy stage) for modalities available and to fabricate and evaluate a generally applicable fetal shield. m...

Chorioamnionitis is a major cause of preterm birth and brain injury. Bacterial invasion of the chorion and amnion, and/or the placenta, can lead to a fetal inflammatory response, which in turn has significant adverse consequences for the developing fetal brain. Accordingly, there is a strong causal link between chorioamnionitis, preterm brain injury and the pathogenesis of severe postnatal neur...