نتایج جستجو برای: fetal calf serum
تعداد نتایج: 393466 فیلتر نتایج به سال:
The nutritional requirements generally satisfied by serum in established cultures of human hematopoietic cells were studied in spinner culture. The optimum concentration of fetal calf serum for cell proliferation in medium RPMI #1640 appeared to be 20%, and the minimum to be 2%. In the logarithmic phase of cell growth, there was an exponential increase of protein in the cells and a correspondin...
A method of freezing cytological material for long term storage, based on a modification of that used in tissue typing for the storage of lymphocytes, was developed. The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological detail was well preserved in a variety ...
Cell culture, radiation exposure and 2D and 3D colony formation assay. Cells were cultured in Dulbecco's Modified Eagle Medium (PAA; plus glutamax-I) supplemented with 10% fetal calf serum (Biochrom) and 1% non-essential amino acids (PAA) (1). Single doses of 200 kV X-rays (Yxlon Y.TU 320; Yxlon; 0.5 mm copper filter; ~1.3 Gy/min, 20 mA) were applied and measured using a Duplex dosimeter (PTW) ...
Apoptosis of fibroblasts may be key for the removal of cells following repair processes. Contraction of three-dimensional collagen gels is a model of wound healing and remodeling. Here two potent inducers of contraction, TGF-beta1 and fetal calf serum (FCS) were evaluated for their effect on fibroblast apoptosis in contracting collagen gels. Human fetal lung fibroblasts were cultured in floatin...
Antibodies to purified calf thymus Fl, and to chromatographically purified subfractions of calf thymus Fl histones were induced by immunization of rabbits with histone-RNA complexes. Complement fixation studies with these antibodies demonstrated structural differences between the subfractions in one organ and between those of different organs and species. The anti-F1 sera showed varying reactiv...
SKG or BALB/c T cells (3.0 £ 10) were stimulated for 72 h with plate-bound anti-CD3 mAb (2C11) in the presence or absence of plate-bound anti-CD28 mAb in RPMI-1640 medium supplemented with 10% fetal calf serum and 50 mM 2-mercaptoethanol. Cells were also stimulated with TPA (1.4 ng ml) and ionomycin (0.14 mM). KJ1.26þ T cells from DO or DO.SKG mice were stimulated with ovalbumin (323–339) pepti...
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