Wheat bran was destarched and deproteinated by a-amylase, protease and amyloglucosidase successively, and further hydrolyzed using Bacillus subtilis xylanases. The yield of enzymatic hydrolysates from wheat bran (EHWB) was 1.84 %. The total phenolics were 0.3712 g of ferulic acid equivalents per gram of EHWB. The antioxidant potency of EHWB was evaluated using different assays, such as iron ion...

Three simple, accurate and economical spectrophotometric methods in ultraviolet and visible region were developed for the determination of cefprozil in bulk and dosage forms have been described. The method A is based on the reduction of ferric ion into ferrous ion by the mentioned drug in the presence of 1,10-phenanthroline to form a highly stable orange red colored ferrion complex measured at ...

The beta-lactamase specified by an R(te16) plasmid in Bordetella bronchiseptica was purified 200-fold by carboxymethyl-Sephadex column chromatography and electrofocusing. The enzyme has a molecular weight of 46,000 +/- 3,000 and an isoelectric point of 8.3 and was highly active against phenethicillin, oxacillin, and propicillin. The enzyme activity was inhibited by sodium chloride but not by fe...

Three distal imidazole pickets in a cytochrome c oxidase (CcO) model form a pocket hosting a cluster of water molecules. The cluster makes the ferrous heme low spin, and consequently the O(2) binding slow. The nature of the rigid proximal imidazole tail favors a high spin/low spin cross-over. The O(2) binding rate is enhanced either by removing the water, increasing the hydrophobicity of the ga...

Adsorption techniques are widely used to remove the pollutants from waters, especially those that are not easily biodegradable. The removal of crystal violet dye (CVD) from water was studied by using Amberlyst 15, a cation-exchange resin (CER), along with Ferrous (Fe 2+ ) and H2O2 . In this study the main factors that can influence the degradation of crystal violet dye are molar ratio of [(H2O2...

Give a brief description of Haemoglobin (Hb):  Hb is made up of Four Subunits (Tetramer) held together by multiple non-covalent interactions  Each subunit (Monomer) consist of: Haem (Ferro-Protoporphyrin) and Globin protein  Haem consist of a Protoporphyrin IX and Ferrous ion (Fe 2 )  Globin protein folds around the Haem group forming a hydrophobic pocket that protects the Haem, which serve...

The radiological properties of nanocomposite Fricke gel (NC-FG) dosimeters prepared with different concentrations of nano-clay, perchloric acid and ferrous ions in deaerated conditions were investigated under carbon and argon ion beam irradiation covering a linear-energy-transfer (LET) range of 10 to 3000 eV/nm. We found that NC-FG exhibits radiological properties distinct from those of convent...

A three-step optimization strategy which includes one-factor-at-a-time classical method and different statistical approaches (Plackett-Burman design and response surface methodology) that were applied to optimize the antioxidant potential of Penicillium granulatum. Antioxidant activity was assayed by different procedures and compared with total phenolic content. Primarily, different carbon and ...

The effects of four widely employed “antioxidants” on iron-mediated hydroxyl radical formation and lipid peroxidation were studied in aqueous model systems. Iron and copper served as catalysts for the reactions which oxidized ascorbic acid and alpha-tocopherol and reduced oxygen. Ferrous ion spontaneously reduced oxygen to 0; (superoxide anion radical) which led to .OH (hydroxyl radical) and Hz...

The aim of this study was to investigate the antioxidant activity using different in vitro assays and nitric oxide synthase activation property of crude water soluble polysaccharide from P. ostreatus. The polysaccharide was isolated by hot water extraction and physico-chemical investigation revealed that it contained high amount of carbohydrate (mostly β-glucan) and low amount of protein and ph...