A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR ...

The classification of hydrocephalus into communicating and non-communicating types is still largely based on the dye test introduced by Dandy and Blackfan in 1914 and 1917. They used the appearance of dye in the spinal fluid after its injection into one of the lateral ventricles as the criterion. In the same papers Dandy and Blackfan described a quantitative urinary dye excretion test. They sho...

zno and tio2 nanoparticles wereimpregnated with merbromin dye and used as modified photocatalysts for degradation of phenol. dye-modified zno and tio2 showed significantly higher photocatalytic activity than neat zno and tio2 under visible light illumination. moreover, the prepared dye-modified zno showed superior photocatalytic efficiency in degradation of phenol compared to the dye-modified t...

In this paper, we propose a two-phase approach to solve a combined routing and scheduling problem that occurs in the textile industry: fabrics are dyed by dye-jets and transported by forklifts. The objective is to minimize the cost of the unproductive activities, i.e., the dye-jet setup times and the forklift waiting time. The first phase solves an integer linear program to assign jobs (fabrics...

This test for systemic lupus erythematosus utilizes a novel liposome composition entrapping the cation-responsive red dye Arsenazo III. In dilutions of normal sera the liposome membranes undergo a rearrangement when divalent cations are added, resulting in the release of the encapsulated dye and the rapid formation of a stable blue cation-dye complex. Microliter amounts of sera from patients wi...

We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...

A simple test for toxic chemicals has been developed. Rhizobium meliloti is combined with the toxic chemical. A tetrazolium dye, MTT (3-[4,5-Dimethylthiazol-2-yl]2,5diphenyl-tetrazolium bromide) is added. The bacterium reduces this dye, causing the optical absorbance to increase dramatically. The increase can be determined with a simple spectrophotometer. Toxic chemicals and minerals inhibit th...

Subfertility is a disease of the reproductive system and defined as the failure to achieve pregnancy after 12 months of unprotected sexual intercourse [1]. Subfertility is a distressing condition for couples who are trying to achieve pregnancy and childbirth. Approximately 15% of couples are affected with subfertility, of which up to 20% remain unexplained. Female factors are identified in abou...