نتایج جستجو برای: fab fragment

تعداد نتایج: 67229  

Journal: :Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2008
David M Goldenberg Edmund A Rossi Robert M Sharkey William J McBride Chien-Hsing Chang

The Dock-and-Lock (DNL) method, which makes bioactive molecules with multivalency and multifunctionality, is a new approach to develop targeting molecules for improved cancer imaging and therapy. It involves the use of a pair of distinct protein domains involved in the natural association between cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) and A-kinase anchoring prote...

Journal: :Journal of virology 2007
John V Williams Zhifeng Chen Gabriella Cseke David W Wright Christopher J Keefer Sharon J Tollefson Ann Hessell Amy Podsiad Bryan E Shepherd Pietro Paolo Sanna Dennis R Burton James E Crowe R Anthony Williamson

Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is a major cause of lower-respiratory-tract disease. hMPV is associated with more severe disease in infants and persons with underlying medical conditions. Animal studies have shown that the hMPV fusion (F) protein alone is capable of inducing protective immunity. Here, we report the use of phage display technology to gene...

Journal: :Journal of virology 2007
Bärbel Kaufmann Alberto López-Bueno Mauricio G Mateu Paul R Chipman Christian D S Nelson Colin R Parrish José M Almendral Michael G Rossmann

The structure of virus-like particles of the lymphotropic, immunosuppressive strain of minute virus of mice (MVMi) in complex with the neutralizing Fab fragment of the mouse monoclonal antibody (MAb) B7 was determined by cryo-electron microscopy to 7-A resolution. The Fab molecule recognizes a conformational epitope at the vertex of a three-fold protrusion on the viral surface, thereby simultan...

Journal: :The Biochemical journal 2013
Nicholas T Redpath Yibin Xu Nicholas J Wilson Louis J Fabri Manuel Baca Arna E Andrews Hal Braley Ping Lu Cheryl Ireland Robin E Ernst Andrea Woods Gail Forrest Zhiqiang An Dennis M Zaller William R Strohl Cindy S Luo Peter E Czabotar Thomas P J Garrett Douglas J Hilton Andrew D Nash Jian-Guo Zhang Nicos A Nicola

Gene deletion studies in mice have revealed critical roles for IL (interleukin)-4 and -13 in asthma development, with the latter controlling lung airways resistance and mucus secretion. We have now developed human neutralizing monoclonal antibodies against human IL-13Rα1 (IL-13 receptor α1) subunit that prevent activation of the receptor complex by both IL-4 and IL-13. We describe the crystal s...

Journal: :Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009
Andrew Want Owen R T Thomas Bo Kara John Liddell Christopher J Hewitt

Microbiology is important to industry therefore rapid and statistically representative measurements of cell physiological state, proliferation, and viability are essential if informed decisions about fermentation bioprocess optimization or control are to be made, because process performance will depend largely on the number of metabolically active viable cells. Samples of recombinant Escherichi...

Journal: :Blood 2009
Sivasubramanian Baskar Jessica M Suschak Ivan Samija Ramaprasad Srinivasan Richard W Childs Steven Z Pavletic Michael R Bishop Christoph Rader

Allogeneic hematopoietic stem cell transplantation (alloHSCT) is the only potentially curative treatment available for patients with B-cell chronic lymphocytic leukemia (B-CLL). Here, we show that post-alloHSCT antibody repertoires can be mined for the discovery of fully human monoclonal antibodies to B-CLL cell-surface antigens. Sera collected from B-CLL patients at defined times after alloHSC...

2014
Rubel Chakravarty Shreya Goel Hector F. Valdovinos Reinier Hernandez Hao Hong Robert J. Nickles Weibo Cai

Scandium-44 (t1/2 = 3.9 h) is a relatively new radioisotope of potential interest for use in clinical positron emission tomography (PET). Herein, we report, for the first time, the room-temperature radiolabeling of proteins with (44)Sc for in vivo PET imaging. For this purpose, the Fab fragment of Cetuximab, a monoclonal antibody that binds with high affinity to epidermal growth factor receptor...

2005
Maarten L. Simoons Harlan F. Weisman

Background Patients with unstable angina despite intensive medical therapy, ie, refractory angina, are at high risk for developing thrombotic complications: myocardial infarction or coronary occlusion during percutaneous transluminal coronary angioplasty (PTCA). Chimeric 7E3 (c7E3) Fab is an antibody fragment that blocks the platelet glycoprotein (GP) Ib/IIIa receptor and potently inhibits plat...

Journal: :The Journal of Cell Biology 1987
B E Bierer S H Herrmann C S Brown S J Burakoff D E Golan

We have studied the lateral mobility of class 1 major histocompatibility complex (MHC) proteins in the membranes of human Epstein-Barr virus-transformed B cells using fluorescence photobleaching recovery. Class I MHC antigens were labeled with either W6/32 monoclonal antibody or its Fab fragment directly conjugated to fluorescein isothiocyanate. The diffusion coefficient of class I antigens lab...

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