The management of wastewater sludge, now often referred to as biosolids, accounts for a major portion of the cost of the wastewater treatment process and represents significant technical challenges. In many wastewater treatment facilities, the bottleneck of the sludge handling system is the dewatering operation. Advanced sludge treatment (AST) processes have been developed in order to improve s...

Extracellular polymeric substances (EPS) play important roles in regulating the dewaterability of sludge. This study sought to elucidate the influence of EPS on the dewaterability of sludge during bioleaching process. Results showed that, in bioleaching system with the co-inoculation of Acidithiobacillus thiooxidans TS6 and Acidithiobacillus ferrooxidans LX5 (A. t+A. f system), the capillary su...

Reactions at ionizable functional groups in extracellular polymeric substances (EPS) from Bacillus subtilis are found to affect aqueous phase conformation and adsorption to mineral surfaces. Characterization by HPSEC, XPS, and FTIR indicates a wide range in apparent molecular mass (0.57-128 kDa), with functional group composition depending on cell growth phase (exponential vs stationary) and lo...

(a) The treatment efficiency in MBR was observed under different aeration rates. It was found that the optimal rate of aeration for removal efficiency was 3m/h.Increasing aeration rate resulted in shear stress, which caused elevated rates of sludge floc breakage, and consequently, supernatant COD. Excessive aeration rate could consequently also affected nitrogen removal by destructing anoxic co...

Extracellular polymeric substances (EPS) are continuously produced by bacteria during their growth and metabolism. In soils, EPS are bound to cell surfaces, associated with biofilms, or released into solution where they can react with other solutes and soil particle surfaces. If such reaction results in a decrease in EPS bioaccessibility, it may contribute to stabilization of microbial-derived ...

Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells. To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, r...

The influence of humic acid and alginate, two major components of natural organic matter (NOM), on deposition kinetics of extracellular polymeric substances (EPS) on silica was examined in both NaCl and CaCl(2) solutions over a wide range of environmentally relevant ionic strengths utilizing a quartz crystal microbalance with dissipation. Deposition kinetics of both soluble EPS and bound EPS ex...

Microbial biofilm formed on abiotic surfaces is an important area of research because of the wide range of possible affects and the disinfectant resistance of the cells. The colonization of solid surfaces by microorganisms is a very complicated process that depends mostly on extracellular molecule production. The biosynthesis of EPS reflected not only the attachment and aggregation process but ...

The sorption of Fe(II) and Fe(III) by extracellular polymeric substances (EPS) of acidophilic bacteria Acidiphilium 3.2Sup(5) and Acidithiobacillus ferrooxidans, harvested from the ecosystem of the Tinto River (Huelva, Spain), was investigated. EPS from mixed cultures of both bacteria (EPS(mixed)) and pure cultures of A. 3.2Sup(5) (EPS(pure)) were extracted with ethylenediamine tetraacetic acid...

Biofilm formation and the production of extracellular polymeric substances (EPS) by meso- and thermoacidophilic metal-oxidizing archaea on relevant substrates have been studied to a limited extent. In order to investigate glycoconjugates, a major part of the EPS, during biofilm formation/bioleaching by archaea on pyrite, a screening with 75 commercially available lectins by fluorescence lectin-...