Ewing sarcoma is the second most frequent pediatric bone tumor. In most of the patients, a chromosomal translocation leads to the expression of the EWS-FLI1 chimeric transcription factor that is the major oncogene in this pathology. Relative genetic simplicity of Ewing sarcoma makes it particularly attractive for studying cancer in a systemic manner. Silencing EWS-FLI1 induces cell cycle altera...

The translocation liposarcoma protein TLS has recently been shown to function as an adapter molecule coupling gene transcription to RNA splicing. Here we demonstrate that YB-1, a protein known to play important roles in transcription and translation, interacts with the COOH-terminal domains of TLS and the structurally related Ewing's sarcoma protein EWS. Through this interaction, YB-1 is recrui...

Ewing sarcomas (ES) are highly malignant bone or soft tissue tumors. Genetically, ES are defined by balanced chromosomal EWS/ETS translocations, which give rise to chimeric proteins (EWS-ETS) that generate an oncogenic transcriptional program associated with altered epigenetic marks throughout the genome. By use of an inhibitor (JQ1) blocking BET bromodomain binding proteins (BRDs) we strikingl...

This work presented economic weights (EWs) calculated for Holstein cattle raised in Slovak dairy productions using a bio-economic approach. EWs are calculated for alternatives with (A) and without (B) market quota. Both alternatives included three market payment systems of milk (base price per milk value was corrected according to content of fat, protein and somatic cells). Marginal EWs for mil...

The term "prodromal symptoms" has traditionally referred to prepsychotic changes in thought, affect, and cognition that precede the initial onset of schizophrenia. Recently, however, the term has been extended into a clinical action context to refer to the early warning signs (EWS) of impending relapse in patients already diagnosed as having schizophrenia. However, recent reports reviewed by No...

Ewing sarcoma (EWS) proto-oncoprotein, an RNA-binding protein, is involved in DNA recombination and repair, gene expression, RNA processing and transport, as well as cell signalling. Chimeric EWS oncoproteins generated by chromosomal translocations between EWSR1 and the genes of transcription factors cause malignant tumors. To understand the loss of function by these translocations, the role of...

BACKGROUND The Dickkopf (DKK) family comprises a set of proteins that function as regulators of Wnt/beta-catenin signaling and has a crucial role in development. Recent studies have revealed the involvement of this family in tumorigenesis, however their role in tumorigenesis is still remained unclear. METHODOLOGY/PRINCIPAL FINDINGS We found increased expression of DKK2 but decreased expressio...

In Ewing sarcoma, NKX2-2 is a critical activated target of the oncogenic transcription factor EWS/FLI that is required for transformation. However, its biological function in this malignancy is unknown. Here we provide evidence that NKX2-2 mediates the EWS/FLI-controlled block of mesenchymal features. Transcriptome-wide RNA sequencing revealed that NKX2-2 represses cell adhesion and extracellul...

An Early Warning System or EWS is a core type of data driven IoT (Internet of Things) system used for environment disaster risk and effect management. The potential benefits of using a semantic type EWS include easier sensor and data source plug-and-play, simpler, richer and more dynamic metadatadriven data analysis and easier service interoperability and orchestration. The challenges faced dur...

Background: Endobronchial embolization is an alternative strategy for the management of pneumothorax or empyema with persistent air leakage. The previous reports analyzed heterogeneous populations of patients, including patients with good performance status (PS, 0 or 1). However, the usefulness of Endobronchial embolization for managing persistent air leakage in patients with poor PS (3 or 4) i...