Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented withdifferent concentrations of N6-benzyladenine (BA) (0.5, 1, ...

BACKGROUND Intact articular cartilage tissue is used clinically in the form of osteochondral allografts and experimentally as explants in modeling the physiologic behavior of chondrocytes in their native extracellular matrix. Long-term maintenance of allograft tissue is challenging. HYPOTHESIS By carefully modulating the preservation environment, it may be possible to preserve osteochondral a...

It has been shown previously that serotonin stimulates the production of prostacyclin by bovine aortic smooth muscle cells in culture, via 5-HT2 receptors (Coughlin SR, Moskowitz MA, Antoniades HN, Levine L. Proc Natl Acad Sci USA 1981;78:7134-7138). These cells express a synthetic phenotype, whereas the majority of the smooth muscle cells in the media from adult arteries are in a contractile s...

BACKGROUND CRC is a significant cause of cancer mortality, and new therapies are needed for patients with advanced disease. TAK-733 is a highly potent and selective investigational novel MEK allosteric site inhibitor. MATERIALS AND METHODS In a preclinical study of TAK-733, a panel of CRC cell lines were exposed to varying concentrations of the agent for 72 hours followed by a sulforhodamine ...

Plant propagation via in vitro culture is a very laborious and time-consuming process. The growth cycle of some of the crop species is slow even in the field and the consistent commercial production is hard to maintain. Enhanced methods of reduced cost, materials and labor significantly impact the research and commercial production of field crops. In our studies, stem-segment explants of Brassi...

In vitro clonal propagation of Ashoka, Saraca asoca (Roxb.) De Wilde plant was achieved using shoot tip, nodal and internodal explants. The explants were cultured on MS medium supplemented with different concentrations (0.5-2.0mg/l) of Benzyl amino purine (BAP), Kinetin (Kn) and 2, 4-Dichlorophenoxy acetic acid (2,4-D). The synergetic effect of BAP (0.5mg/ l) induced a mean of 11.71±0.53 advent...

In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 microM), Kinetin (KN-2.32, 4.65, and 9.29 microM) and Coconut water (CW, 5, 10 and 15%) individually or in combination with 2.69 m...

Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404 strain) was performed in two ways: in vitro by the transformation of root and stem explants and inplanta by the transformation of whole plants in the green­ house. Agrobacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that tra...

A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...

Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and i...