Exogenous polyribonucleotides stimulated the ribonucleic acid (RNA) transcriptase in Sendai virions. Added yeast RNA, polyadenylic acid, or polycytidylic acid increased incorporation of (3)H-guanosine monophosphate as much as fivefold. The products of stimulated reactions were virus-specific as determined by hybridization with Sendai virion RNA, but they sedimented more slowly (13s) than the pr...

The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxylase (ODC) activity. The results suggest that diminished OppA content, but not...

A series of alkylated (bis)urea and (bis)thiourea polyamine analogues were synthesized and screened for antimalarial activity against chloroquine-sensitive and -resistant strains of Plasmodium falciparum in vitro. All analogues showed growth inhibitory activity against P. falciparum at less than 3 μM, with the majority having effective IC(50) values in the 100-650 nM range. Analogues arrested...

l-Arginine; Anti-aging; Clinical pharmacology; Abstract l-Arginine is one of the most metabolically versatile amino acids. In addition to its role in the synthesis of nitric oxide, l-arginine serves as a precursor for the synthesis of polyamines, proline, glutamate, creatine, agmatine and urea. Several human and experimental animal studies have indicated that exogenous l-arginine intake has mul...

Spermidine is rapidly taken up and becomes bound to protein during the very early hours of sea urchin embryogenesis. During the first 6 hr after fertilization of freshly obtained sea urchin eggs (Strongylocentrotus purpuratus), which are incubated in the presence of exogenous [3H]-spermidine, up to 7% of the total cell-associated spermidine appears uniquely as spermidine bound in macromolecular...

A high performance liquid chromatographic (HPLC) method, based on dansylation and fluorescence detection, is described for the estimation of putrescine, spermidine, and spermine in lichen (Evernia prunastri [L.]) samples. Because of the high concentrations of phenols and salts, dansylation was followed by a pre-HPLC purification step. Both flow rate and mobile phase (methanol:water) followed a ...

This perspective paper proposes that endogenous apoplastic fructans in fructan accumulating plants, released after stress-mediated cellular leakage, or increased by exogenous application, can act as damage-associated molecular patterns (DAMPs), priming plant innate immunity through ancient receptors and defense pathways that most probably evolved to react on microbial fructans acting as microbe...

heavy metal pollution is a worldwide problem with serious environmental consequences. the objective of the present experiment was to investigate whether arginine as nitric oxide precursor and or polyamines substrate can decrease the destructive effects of oxidative stress induced by nickel contamination in hyoscyamus niger plant. in this study the effects of arginine pretreatment on alkaloid co...

In order to investigate the seasonal changes and the possible role of the free polyamines in the leaves and stems on the alternate bearing habit of the ‘Kinnow’ mandarin (Citrus reticulata Blanco) trees, a comparative study was conducted to analyze the levels of free polyamines  (putrescine, spermidine and spermine) in the leaves and stems tissues of “on” and &ldquo...

Background: The application of polyamines as a plant growth substance with effect on a wide range of plant growth and development processes include cell division, flowering, senescence delay, membrane stability, absorb active radicals and different stress tolerance and polyamines Function as a anti-senescence and anti-stress agents in the plant and on the other hand the competitiveness of their...