Feed ingredients of plant origin are commonly used in swine diets. However, the major components of plant cell walls, non-starch polysaccharides (NSPs), reduce nutrient digestibility. To improve the efficiency of feed utilization, exogenous enzyme products that degrade NSPs have been widely used in commercial animal feeds. Nonetheless, the effects of exogenous enzyme addition to swine diets on ...

Sheep pulmonary adenomatosis ([SPA] ovine pulmonary carcinoma) is a transmissible lung cancer of sheep that has been associated etiologically with a type D- and B-related retrovirus (jaagsiekte retrovirus (JSRV]). To date it has been impossible to cultivate JSRV in vitro and therefore to demonstrate the etiology of SPA by a classical approach. In addition, the presence of 15 to 20 copies of end...

Strigolactone (SL) plays essential roles in regulating plant growth, development, and stress response. This study was conducted to evaluate the effect of exogenous SL on watermelon resistance against herbicides penoxsulam (PXL) bensulfuron-methyl (BSM). These were found negatively impact root growth photosynthetic pigments, probably due ultrastructural damage cell death leaf tissues under herbi...

As an important reserve land resource, beachfront mudflats have been extensively reclaimed over the past few decades. In recent years, more and research has begun to close Note improvement of overall environmental quality soil, including soil biological properties, in process beach utilization. Among them, enzymes are activities The activity level product is a good indicator quality, which can ...

A rat brain Golgi sialyltransferase activity capable of the differentiation-dependent control of N-CAM sialylation state is described. The specific activity of Golgi sialyltransferase was found to be developmentally regulated with respect to both endogenous and exogenous protein acceptors, with a particular elevation on postnatal days 10-12 when the heavily sialylated or 'embryonic' form of N-C...

Neural retinal cells from 9-d-old chick embryos were assayed for uridine diphosphate (UDP)-galactose:ganglioside GM2 galactosyltransferase, or GM1 synthetase, activity using the oligosaccharide fragment of GM2, oligo-GM2, oligo-GM2, as the exogenous acceptor. The results demonstrated that this enzyme activity was present on the external surfaces of intact cells. Little difference between the sp...

A true Brønsted analysis of proton transfer in an enzyme mechanism is made possible by the chemical rescue of an inactive mutant of aspartate aminotransferase, where the endogenous general base, Lys258, is replaced with Ala by site-directed mutagenesis. Catalytic activity is restored to this inactive mutant by exogenous amines. The eleven amines studied generate a Brønsted correlation with beta...

Enzyme replacement has been under consideration as a therapeutic strategy for patients with Gaucher disease for more than two decades. Previous studies indicated that single injections of purified glucocerebrosidase reduced the amount of storage material in the liver. It was important to determine whether administration of exogenous enzyme on a regular basis would be of clinical benefit. We rep...

The enzymic incorporation of choline-l,2-'C from CDP-choline-1 ,V4C into phosphatidylcholine by spinach leaf preparations was characterized. The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required either Mn2+ or Mg2+ as cofactor. The saturation concentration of Mn2+ was 0.3 mM and that for Mg2+ was 13 mM. The nT, for CDP-choline was 10 p ~...

In order to reveal the effect of exogenous neutral protease addition on quality cigar tobacco leaves during fermentation. Taking Sichuan Shifang as research object, changes conventional chemical composition, amino acid content, organic acids and other substances in after natural fermentation with enzyme preparations were investigated, sensory before also investigated. The results show that: 1) ...