Ten strains of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule of soybean, which were isolated from various soybean growing regions of Thailand, produced an extracellular diffusible factor (DSF) related to a well-characterized quorum sensing molecule produced by other Xanthomonas spp. Genomic DNA of the 10 strains of X. axonopodis pv. glycines contained rpfF, a gene e...

The genes encoding two different phospholipase C exoenzymes of Bacillus cereus are directly linked. A sequence comprising the gene for phospholipase C and part of the sphingomyelinase C (SMase) gene from strain SE-1 (1) as well as the entire SMase gene sequence from strain IAM 1208 (2) have recently been determined. We now report the complete sequence of the SMase gene from strain SE-1. Surpris...

C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and thereby inactivates Rho A, B and C GTPases in mammalian cells. The structure of a tetragonal crystal form has been determined by molecular replacement and refined to 1.89 A resolution. It is very similar to the apo structures determined previously from two different monoclinic crystal forms. An objective reassessment of availa...

PURPOSE The exoenzyme S regulatory protein ExsA regulates a type III secretion system in Pseudomonas aeruginosa. In vitro, cytotoxic strains use this system to secrete exotoxin (Exo)U and ExoT causing cytotoxicity and inhibiting their phagocytosis by epithelial cells. Invasive P. aeruginosa secrete ExoT and ExoS, but exsA mutation has little impact on their short-term interactions with epitheli...

CPI-17 (Protein kinase C potentiated phosphatase inhibitor of 17 KD) mediates some agonist-induced smooth muscle contraction by suppressing the myosin phosphatase in a phosphorylation-dependent manner. Physiological relevant kinase(s) that phosphorylate CPI17 remains to be identified. Several previous studies have shown that some agonist-induced CPI-17 phosphorylation in smooth muscle tissues w...