-Short-chain saturated fatty acids (SCSFA), ranging from pentanoic (C5) to decanoic acid (C~0), when added at micromolar concentrations to the germination medium of chick-pea seeds, did not significantly affect radicle emergence, although fresh weight diminished. Only heptanoic acid (C7) and octanoic acid (Cs) stimulated ethylene production and free spermidine and spermine content, whereas putr...

Adventitious root (AR) formation in the stem base (SB) of cuttings is the basis for propagation of many plant species and petunia is used as model to study this developmental process. Following AR formation from 2 to 192 hours post-excision (hpe) of cuttings, transcriptome analysis by microarray revealed a change of the character of the rooting zone from SB to root identity. The greatest shift ...

This paper uses self-assembled monolayers on gold as a model system to demonstrate that the attachment and spreading of Swiss 3T3 fibroblasts depends strongly on the microenvironment of immobilized RGD peptides. This work utilized monolayers that present mixtures of Arg-Gly-Asp peptides, which are ligands for cellular integrin receptors, and oligo(ethylene glycol) groups, which resist the nonsp...

The detection of human serum albumin (HSA) is significant clinical importance in disease diagnoses. In this work, polymer-based synthetic receptors are designed by incorporating Ag-ZnS microspheres molecularly imprinted poly(methacrylic acid-co-ethylene glycol dimethacrylate) (MIPs) for the gravimetric HSA. Among different compositions Ag-ZnS@MIPs, MIPs having methacrylic acid and ethylene dime...

A fuel-cell-type gas sensor based on the membrane electrode assemble (MEA) composed of proton exchange membrane (Nafion) and Pt/C sensing electrodes was fabricated for detection of CO at room temperature. The Pt/C electrodes materials were synthesized from Vulcan XC-72 carbon powders and hexachloroplatinic acid (H2PtCl6). This paper mainly focused on the effect of various dispersants (ethylene ...

Potato tubers (Solanum tuberosum cv. Sebago) were stored at 20 °C in air containing ethylene at <0.005, 0.01, 0.1, 1.0, or 10 μL·L–1 and the level of sprouting was measured over 35 days. The time for tubers to develop an average of one sprout per tuber was found to linearly increase as the log10 ethylene concentration decreased with the effect present over the whole range of concentration. Afte...

Arabidopsis AtTRP1 is an orthologue of SlTPR1, a tomato tetratricopeptide repeat protein that interacts with the tomato ethylene receptors LeETR1 and NR in yeast 2-hybrid assays and in vitro, and modulates plant development. AtTRP1 is encoded by a single copy gene in the Arabidopsis genome, and is related to TCC1, a human protein that competes with Raf-1 for Ras binding, and distantly related t...

The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1) gene in transgenic Centaurium erythraea. The optimization of the PCR protocol involved a gradient of annealing temperatures, as well as the applica...

Novel complexation hydrogel nanospheres of poly(methacrylic acid-grafted-poly(ethylene glycol)) (P(MAA-gEG)) were prepared by dispersion polymerization to be used for protein delivery applications. Polymerization was conducted in solvents such as deionized water, ethanol/water, sodium hydroxide, hydrochloric acid, and acetic acid solutions. When polymerizing in deionized water we produced nanos...