Mechanical load and postmenopausal hypoestrogen are risk factors for pelvic organ prolapse (POP). In this study, we applied a 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10⁻⁸ M 17-β-estradiol to vaginal fibroblasts isolated from postmenopausal women with or without POP to investigate the effects of CS and estrogen on cell morphology and cytoskeletons of normal and ...

Pre-treatment of human lymphocytes with 17 beta-estradiol diminishes the increase in concentration of cytosolic free calcium after stimulation with phytohaemagglutinin. The effect is dependent on 17 beta-estradiol concentration and on the preincubation time. The effect is not due to an interaction between 17 beta-estradiol and phytohaemagglutinin, but appears to be a consequence of the binding ...

The influence of estrous cycle and intrastriatal implants of 17 beta-estradiol (17 beta-estradiol). 17 alpha-estradiol (17 alpha-estradiol) or cholesterol on the number of footfaults made by female rats traversing a narrow suspended beam was investigated. Female rats made fewer footfaults on estrus than on other days of the cycle. This was true when testing occurred during either the light or d...

Although the ovaries produce majority of estrogens in women before menopause, estrogen is also synthesized peripheral tissues such as adipose tissue (AT). The typical female AT distribution, concentrated subcutaneous and femoro-gluteal regions, estrogen-mediated, but significance synthesis premenopausal poorly understood. Serum visceral homogenates from 28 undergoing non-malignant surgery were ...

The presence of steroid hormones in lake waters causes contamination aquatic ecosystems, which may cause endocrine alterations the organisms that inhabit them. Moreover, many these are purified and distributed to populations located around lake. Therefore, effects could be repeated humans consuming water. This study reports hormone residues inner bay Lake Titicaca drinking water city Puno (Peru...

Mammalian steroid hormones (MSH) are also inherently synthesized by several plant species. However, external application of further stimulates cell division, pollen germination, growth and development. There is little known about the effects MSH on polymorphism DNA damage in Triticum aestivum L under vitro conditions. In this study, inter-primer binding site (iPBS) retrotransposon coupled restr...

We describe the preparation of [2,4,16,16-2H4]estradiol-17 beta. The method for quantitative determination of subpicomole amounts of estradiol-17 beta (trimethylsilyl ether derivatives) by gas-liquid chromatography-mass spectrometry with multiple ion detector and with [2H4]estradiol-17 beta as the carrier is presented and characterized. Values for serum samples, as measured by radioimmunoassay ...

Objective: A simple high-performace liquid chromatography method was developed and validated to determine 17-β estradiol in poly (ε-caprolactone) nanocapsules.
 Methods: The chromatographic conditions were as follows: C18 GL column with a mobile phase of acetonitrile:water (92:8 v/v) at flow rate 1.5 mL/min detection 280 nm. evaluated parameters specificity, linearity, limits quantificatio...