FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

Our work is based on the premise that analysis of the connections exploited by a recommender algorithm can provide insight into the algorithm that could be useful to predict its performance in a fielded system. We use the jumping connections model defined by Mirza et al. [6], which describes the recommendation process in terms of graphs. Here we discuss our work that has come out of trying to u...

suggests mohammed arcon - researcher algeria at the university of sorbonne - new approaches to the understanding and interpretation of the koran are alternative approaches muslim commentators and has views of strange in some verses of the koran contradicts what muslims are unanimously agreed among his views interpreted the meaning and erased mentioned in verse twelve nisa hamaah any wife of a s...

We report the kindred with familial isolated hyperparathyroidism with parathyroid cancer. The proband was diagnosed as having primary hyperparathyroidism at age 43. The same disorder was also found in his daughter who had low bone mass. His son was found to have primary hyperparathyroidism by family screening. The pathological diagnosis of the resected parathyroid in both father and daughter wa...

other persons who are mentioned because at some time something has been named after them. Historians may hope that future editions will not prune too much on the ground of obsolescence. Manifestly there is no outright "best buy": Firkin & Whitworth contain material not to be found elsewhere, and similarly much that they do not cover is available elsewhere. All depends on what you seek. They can...

ajp.psychiatryonline.org order to get him back on the road. In addition, he was generally very punctual but seemed to exhibit a new disregard for time. For example, he typically awakened early to take his son to school, but 2 days prior to admission he overslept, displaying an uncharacteristic apathy about getting his son to school on time. Upon psychiatric admission, prazosin was discontinued....