Posaconazole, indicated for prophylaxis of invasive Aspergillus and Candida infections in immunosuppressed patients aged 13 years or older and for treatment of oropharyngeal candidiasis (Table 1), is like other triazole antifungals in that it blocks ergosterol biosynthesis. 1 Its chemical structure is most similar to that of itraconazole (Figure), which may confer efficacy even against strains ...

Posaconazole and itraconazole were more potent inhibitors of ergosterol synthesis, in both intact cells and cell extracts from Absidia corymbifera and Rhizopus oryzae, than voriconazole and fluconazole. Similarly, expression of CYP51 from R. oryzae in Saccharomyces cerevisiae significantly increased resistance to fluconazole and voriconazole but not to posaconazole and itraconazole.

Nystatin (5 to 10 mug/ml) was fungicidal to Saccharomyces cerevisiae NCYC 361. There was an initial rapid uptake of nystatin by the yeast cells at 30 C, the uptake being pH dependent, with a maximum at low pH values (3 to 4). Photomicrographs indicated that nystatin-treated cells became granular. The effect of growth temperature on the subsequent sensitivity of the yeast cells to nystatin was i...

Airborne and waterborne fungal spores were compared with respect to cytoplasmic viscosity and the presence of ergosterol. These parameters differed markedly between the two spore types and correlated with spore survival. This suggests that the mode of spore dispersal has a bearing on cellular composition, which is relevant for the eradication of industrially relevant fungal propagules.

Symbiotic interactions between microorganisms and social insects have been described as crucial for the maintenance of these multitrophic systems, observed stingless bee Scaptotrigona depilis yeast Zygosaccharomyces sp. SDBC30G1. The larvae S. ingest fungal filaments SDBC30G1 to obtain ergosterol, which is precursor biosynthesis ecdysteroids that modulate insect metamorphosis. In this work, we ...

UNLABELLED Ergosterol is a major and specific component of the fungal plasma membrane, and thus, the cytochrome P450 enzymes (Erg proteins) that catalyze ergosterol synthesis have been selected as valuable targets of azole antifungals. However, the opportunistic pathogen Aspergillus fumigatus has developed worldwide resistance to azoles largely through mutations in the cytochrome P450 enzyme Cy...

THIS paper describes a continuation of the work of Rosenheim and Adam [1929] on unimolecular films of derivatives of ergosterol, spread on a water surface. Its object is to obtain information as to the properties of the molecules in these films, in the hope of throwing fresh light on the constitution of the various products. While some progress has been made, there are still many points in the ...

We investigated the stereoselective inhibition of growth and ergosterol biosynthesis by SCH39304 in the pathogenic fungus Cryptococcus neoformans obtained from four AIDS patients who failed fluconazole therapy and compared the results to those obtained with a wild-type strain. For all strains, the MICs of the RR isomer were approximately half those of the racemate, with the SS enantiomer showin...

Rice planthoppers and anobiid beetles harbor intracellular yeastlike symbiotes (YLS), whose sterols are nutritionally advantageous for the host insects that cannot synthesize sterols. YLS of anobiid beetles synthesize ergosterol, whereas YLS of planthoppers produce ergosta-5,7,24(28)-trienol, which is a metabolic intermediate in the ergosterol biosynthetic pathway in yeasts. Since sterol C-22 d...