A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.

1 Department of Neurology, Medical Faculty, Heinrich-Heine Universität, Düsseldorf, Germany 2 Institute for Medical Biochemistry and Molecular Biology, University Medicine, Ernst-Moritz-Arndt Universität, Greifswald, Germany 3 Departamento de Bioquímica, Universidad de la República, Montevideo, Uruguay 4 Department of Chemistry, University of Padova, Padova, Italy *Correspondence: carsten.bernd...

Lignocellulose is the most abundant carbohydrate source in nature and represents an ideal renewable energy source. Thermostable enzymes that hydrolyze lignocellulose to its component sugars have significant advantages for improving the conversion rate of biomass over their mesophilic counterparts. We review here the recent literature on the development and use of thermostable enzymes for the de...

Metagenomics has accelerated the process of discovery of novel biocatalysts by enabling scientists to tap directly into the entire diversity of enzymes held within natural microbial populations. Their characterization has revealed a great deal of valuable information about enzymatic activity in terms of factors which influence their stability and activity under a wide range of conditions. Many ...

the effect of a neuropeptide, the tachykinin on α-amylase, protease and lipase activities in the midgut of american cockroach, periplaneta americana (linnaeus) is evaluated . immunohistochemical reactivities against tachykinin (tachykinin- ir) were detected in the midgut. tachykinin-ir cells in three regions (anterior, median and posterior) of the midgut were counted in normally fed, starved fo...

this study was performed to investigate the effect of ag+ as an elicitor on the activity of antioxidant enzymes, content of h2o2, accumulation of total tocopherol and antioxidant activity in hairy root cultures of silybum marianum (l.) gaertn. the hairy roots were treated with 2 mm ag+. the treated and non-treated hairy roots were harvested in the same time and data on h2o2 content, dpph, tocop...

background: there is much evidence indicating that natural substances from edible and medicinal plants possess powerful antioxidant and hepatoprotective activities. the objective of the present study was to investigate the potential hepatoprotective effect of silymarin in fish exposed to malathion. methods: zebra cichlid fish were allocated into five groups of which one group received normal fe...

conclusions the results suggest that physical activity improves metabolic parameters, which interfere in the development of fatty liver and has a protective role against the development of nafld. results statistical analysis demonstrated that the training group had significant changes in weight, bmi, tc, tg, ldl, vldl, hdl/ldl, tc/ hdl, ast and alt, whereas there was no significant change in hd...

the present study investigated the effects of e. coli lipopolysaccharide (lps) induced mastitis model in rat on the activity of antioxidant enzyme systems. to achieve this purpose, e. coli lps were infused into the mammary gland of 12 rats 72 hr postpartum and compared with 12 rats in control group infused intramammary placebo sterile pyrogene – free, physiological saline. the antioxidant activ...