Escherichia coli strains are among the major bacterial causes of diarrheal illness. At least 5 categories of diarrheagenic E. coli (DEC) are recognized, namely enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), and enterohemorrhagic E. coli (EHEC). Due to the need for costly and labor-intensive diagnostic procedures,...

Foodborne diseases occur worldwide, including those acquired through the consumption of contaminated meat. This study aimed to investigate the prevalence of enterotoxigenic Staphylococcus aureus, Salmonella and Escherichia coli pathotypes in different meat types. Forty meat samples fresh (n=35) and frozen (n=5) were purchased from local markets in Jenin district, Palestine. Multiplex PCR was us...

diarrheagenic escherichia coli have developed different strategies for establishment of infection in their host. understanding these pathogenic mechanisms has led to the development of specific diagnostic tools for identification and categorization of e. coli strains into different pathotypes. this review aims to provide an overview of the various categories of diarrheagenic escherichia coli an...

Although part of the normal gut flora, Escherichia coli strains are also important intestinal pathogens. Diarrheagenic E. coli (DEC) cause a wide variety of gastrointestinal diseases, particularly among children in developing countries, resulting in significant morbidity and mortality (Moyo, 2007; Khairun, 2007; Brandal, 2007; Nguyen, 2005; Rajendran, 2010; Franzolin, 2005). The DEC are differe...

Diarrheagenic Escherichia coli (DEC) is important bacteria of children's endemic and epidemic diarrhea worldwide. The aim of this study was to determine the prevalence of DEC isolated from stool samples collected from children with acute diarrhea living in Ouagadougou, Burkina Faso. From August 2013 to October 2015, stool samples were collected from 315 children under 5 years of age suffering f...

We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs ...

BACKGROUND Diarrheal diseases can be caused by viral, bacterial and parasitic infections. This paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in Tunisian children less than five years of age. METHODS Overall, 124 diarrhoeal stools were collected from patients suffering from acute diarrhea and 54 stool samples from healthy children. All stools...

The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...

This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAggEC), enteropathogenic E...