The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90°C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the gener...

Background: Developing and promoting empathy between couples can assist them in dealing with destructive communication patterns, increasing their satisfaction through effective dialogue and conversation, and, as a result, creating a safe place to meet emotional needs. The present study aimed to determine the effectiveness of Olson’s enrichment training program and Johnson’s relationship enrichm...

Genome-wide association studies (GWASs) have mined many common genetic variants associated with human complex traits like diseases. After that, the functional annotation and enrichment analysis of significant SNPs are important tasks. Classic methods are always based on physical positions of SNPs and genes. Expression quantitative trait loci (eQTLs) are genomic loci that contribute to variation...

Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...

SELEX (systematic evolution of ligands by exponential enrichment) is a very powerful method for determining the binding site of a protein on RNA. It relies on the ability of an RNA-binding protein to select high-affinity RNA ligands from a randomized pool of RNAs. SELEX experiments are performed over several "rounds," with each round resulting in increased enrichment of RNAs capable of binding ...

We demonstrate a method of improving a seed sentiment lexicon developed on essay data by using a pivot-based paraphrasing system for lexical expansion coupled with sentiment profile enrichment using crowdsourcing. Profile enrichment alone yields up to 15% improvement in the accuracy of the seed lexicon on 3way sentence-level sentiment polarity classification of essay data. Using lexical expansi...

as igm and iga-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of igm and iga enriched immunoglobulins. fraction iii which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using sephadex deae a-50 and 0.1 m tris-d.35m naci buff...

in this study the optimal amount of enriching artemia franciscana with a synbiotic combination of pediococcus acidilactici and fructooligosaccharide (fos( was investigated. the experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics p. acidilactici (1×109, 1×108 and 1× 107 cfu per l) and multi-level enriched prebiotic fos (1, 2 and 5) g per l of solu...

the present study investigates the possibility of enriching adult artemia franciscana with singular or combined administration of pediococcus acidilactici and fructooligosaccharide (fos). the experiment was conducted in a completely randomized design with four treatments, including synbiotic, p. acidilactici +fos (t1), probiotic, p. acidilactici (t2), prebiotic, fos (t3) and control (t4). to ev...

This work presents an efficient and selective enrichment method for glycoprotein/glycopeptides with sulfonic acid-functionalized mesoporous silica (SBA-15-SO3H), which is synthesized via simple oxidation of -SH groups with H2O2. The functionalized SBA-15 shows large surface area and accessible pores, and can selectively adsorb glycopeptides via hydrogen bond and hydrophilic interaction. Upon th...