in spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be pa...

Stem cells are undifferentiated cells that are capable of dividing for long periods of time and can give rise to specialized cells under particular conditions. Embryonic stem cells [5] are a particular type of stem cell derived from embryos. According to US National Institutes of Health [6] (NIH), in humans [7], the term ?embryo? applies to a fertilized egg [8] from the beginning of division up...

Since drawing the attention of scientists, ethicists, and policymakers, stem cells have not lost their place as one of the most promising yet controversial scientific discoveries of the 20th century. Scientists admit that they still know very little about how these undifferentiated pluripotent precursors work, but they're looking to develop a more sophisticated view, one that will unlock stem c...

Naïve pluripotent mouse embryonic stem cells (ESCs) resemble the preimplantation epiblast and efficiently contribute to chimaeras. Primate ESCs correspond to the postimplantation embryo and fail to resume development in chimaeric assays. Recent data suggest that human ESCs can be 'reset' to an earlier developmental stage, but their functional capacity remains ill defined. Here, we discuss how t...

background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...