The zona pellucida (ZP) is a dynamically changing object that plays an important role during the preimplantation stage of embryogenesis. ZP thickness may affect implantation success and pregnancy rate, it considered as prognostic factor in number studies. study was aimed to assess dynamic changes mouse embryonic after laser assisted hatching (LAH) involved breaching integrity at blastocyst stag...

Abstract Hydrosalpinx is a disease characterized by the obstruction of salpinx, with progressive accumulation in shape fluid-filled sac at distal part tuba uterina, and closed to ovary. Women hydrosalpinges have lower implantation pregnancy rates due combination mechanical chemical factors thought disrupt endometrial environment. Evidence suggests that presence hydrosalpinx reduces rate assiste...

Transplantation therapy for diabetes is limited by unavailability of donor organs and outcomes complicated by immunosuppressive drug toxicity. Xenotransplantation is a strategy to overcome supply problems. Implantation of tissue obtained early during embryogenesis is a way to reduce transplant immunogenicity. Insulin-producing cells originating from embryonic pig pancreas obtained very early fo...

Cell types more advanced in development than embryonic stem cells, such as EpiSCs, fail to contribute to chimeras when injected into pre-implantation-stage blastocysts, apparently because the injected cells undergo apoptosis. Here we show that transient promotion of cell survival through expression of the anti-apoptotic gene BCL2 enables EpiSCs and Sox17+ endoderm progenitors to integrate into ...

The number of kidney allotransplants performed per year is limited by the availability of human donor organs. Xenotransplantation of a vascularized organ, such as the kidney, as an alternative to allotransplantation presents formidable immunological challenges. One novel solution to this conundrum is to use embryonic kidneys taken from animal donors early during organogenesis when metanephroi c...

Motivation Embryonic development is a highly complex process that requires precise spatial and temporal regulations. Through the preimplantation stages, single-cell zygotes undergo drastic gene expression changes to form multicellular blastocysts. Proper blastocyst formation is crucial to the success of fetus development, as poor blastocyst quality often lead to failure in post-in vitro fertili...

The closely related mitogen-activated protein kinase isoforms extracellular signal-regulated kinase 1 (ERK1) and ERK2 have been implicated in the control of cell proliferation, differentiation and survival. However, the specific in vivo functions of the two ERK isoforms remain to be analysed. Here, we show that disruption of the Erk2 locus leads to embryonic lethality early in mouse development...

Transplantation therapy for diabetes is limited by unavailability of donor organs and outcomes complicated by immunosuppressive drug toxicity. Xenotransplantation is a strategy to overcome supply problems. Implantation of tissue obtained early during embryogenesis is a way to reduce transplant immunogenicity. Insulin-producing cells originating from embryonic pig pancreas obtained very early fo...

This paper considers embryo grading within a given infertility treatment and suggests an ethical approach to embryo donation for embryonic stem cell harvest. It is concluded that ethical considerations regarding human embryos do not necessarily preclude the use of certain embryos for biomedical research or transplantation. The argument is based on the following rationale: all embryos are not ph...

The Sin3a/HDAC co-repressor complex is a critical regulator of transcription networks that govern cell cycle control and apoptosis throughout development. Previous studies have identified Sin3a as essential for embryonic development around the time of implantation, during which the epiblast cell cycle is uniquely structured to achieve very rapid divisions with little tolerance of DNA damage. Th...