In order to detect and quantify the phenolic contents of a wastewater with biosensors, two working electrodes based on modified Poly(Pyrrole) films were fabricated. Enzyme horseradish peroxidase was used as biomolecule of the prepared electrodes. Various phenolics were tested at the biosensor. Phenol detection was realized by electrochemical reduction of quinones produced by enzymatic activity....

PURPOSE A significant barrier to efficient antibiotic management of infection is that the standard diagnostic methodologies do not provide results at the point of care. The delays between sample collection and bacterial culture and antibiotic susceptibility reporting have led to empirical use of antibiotics, contributing to the emergence of drug resistant pathogens. As a key step toward the dev...

This study demonstrates the application feasibility of electrochemical impedance spectroscopy (EIS) in measuring estrogen (17β-estradiol) in gas phase. The present biosensor gives a linear response (R2 = 0.999) for 17β-estradiol vapor concentration from 3.7 ng/L to 3.7 × 10-4 ng/L with a limit of detection (3.7 × 10-4 ng/L). The results show that the fabricated biosensor demonstrates better det...

The bovine serum albumin (BSA) protein is employed in genosensors as nonspecific binding blocker. In this work, the influence of BSA on electrochemical response a DNA-based biosensor explored by impedance spectroscopy (EIS) and double pulse voltammetry technique. Each buffer component hybridization studied bare gold electrodes single-stranded DNA (ssDNA)-functionalized electrodes. found to have...

An Au-on-Au tip sensor is developed for the detection of Salmonella typhimurium (Salmonella), using a new synthetic nucleic acid probe (NAP) as linker immobilization DNA-conjugated Au nanoparticle (AuNP) onto DNA-attached thin layer inside pipette tip. In presence Salmonella, RNase H2 from (STH2) cleaves NAP and freed AuNP can be visually detected by paper strip. This portable biosensor does no...

Novel spherical Pd@Cys-C(60) nanoparticles were synthesized using an in situ spontaneous reduction process without any other reducing agent. A nonenzymatic electrochemical biosensor was developed for the detection of glucose based on the spherical nanoparticles film.

The development of high sensitivity biosensors, for example for clinical diagnostics, requires the identification of suitable receptor molecules which offer high stability, specificity and affinity, even when embedded into solid-state biosensor transducers. Here, we present an electrochemical biosensor employing small synthetic receptor proteins (Mw < 15 kDa) which emulate antibodies but with i...

An efficient electrochemical approach is developed for ultrasensitive profiling of the methylation status of the p53 tumor suppressor gene based on a label-free biosensor in combination with bisulfite conversion.

An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for b...

Abstract As a “star material”, 2D transition metal carbides and/or nitrides (MXenes) have tremendous potential applications in biosensor development and molecular imaging. MXenes lot of advantages due to their large specific surface, excellent electrical conductivity, adjustable band gap, easy modification. that immobilized with DNA strands, proteins, enzymes, or other bioluminescent materials ...