A 30-kDa major outer membrane protein of Ehrlichia canis, the agent of canine ehrlichiosis, is the major antigen recognized by both naturally and experimentally infected dog sera. The protein cross-reacts with a serum against a recombinant 28-kDa protein (rP28), one of the outer membrane proteins of a gene (omp-1) family of Ehrlichia chaffeensis. Two DNA fragments of E. canis were amplified by ...

In our experience with Greyhounds over the past 17 years, the prevalence of TBDs has decreased markedly, likely due to better tick control. For example, 15 years ago approximately half of the retired racing Greyhounds evaluated as potential blood donors at OSU tested positive for Babesia canis or Ehrlichia canis serology, whereas now only 1-3% of the dogs are positive for any TBD. However, in s...

A Leishmaniose Visceral Canina (LVC) pode servir como diagnóstico diferencial para Erliquiose ou Babesiose Canina, hemoparasitoses transmitidas por carrapatos, dada a similaridade das manifestações clínicas e urbanização do vetor da LVC. Neste relato de caso, apontamos importância se ter no histórico cão atendido região em que ele vive. O relatado vinha Itu-SP apresentava apenas epistaxe unilat...

Dogs acquire infections with the Anaplasmataceae family pathogens, E. canis, E. chaffeensis, E. ewingii, A. platys and A. phagocytophilum mostly during summer months when ticks are actively feeding on animals. These pathogens are also identified as causing diseases in people. Despite the long history of tick-borne diseases in dogs, much remains to be defined pertaining to the clinical and patho...

A total of 344 dogs belonging to people in resource-poor communities in North West Province, South Africa, was examined for ectoparasites, and all visible arthropods were collected from the left side of each dog. By doubling these numbers it was estimated that the dogs harboured 14,724 ixodid ticks, belonging to 6 species, 1,028 fleas, belonging to 2 species, and 26 lice. Haemaphysalis leachi a...

Ehrlichia canis is a small pleomorphic gram-negative, coccoid, obligatory intracellular bacterium and the cause of canine monocytic ehrlichiosis. A real-time fluorescence resonance energy transfer polymerase chain reaction (real-time FRET PCR) coupled with melting curve analysis was established for detection of E. canis infection in canine blood samples. The VirB9 gene was amplified using one p...

Ehrlichia canis (E. canis) is a lipopolysaccharide (LPS)-deficient obligatory intracellular bacterium that causes canine monocytic ehrlichiosis, a chronic febrile disease accompanied with hematological abnormality. This study analyzed temporal expression levels of IL-1beta, IL-2, IL-6, IL-8, IFN-gamma, and TNF-alpha mRNA by peripheral blood leukocytes from dogs experimentally infected with a ne...