BACKGROUND Site-selective modification of proteins at two separate locations using two different reagents is highly desirable for biosensor applications employing fluorescence resonance energy transfer (FRET), but few strategies are available for such modification. To address this challenge, sequential selective modification of two cysteines in glucose/galactose binding protein (GGBP) was demon...

in this research, the removal of malachite green dye from textile wastewaters using polyaniline coated onto sawdust (pani/sd) has been investigated. ammonium peroxodisulphate was used as chemical oxidant for polymerization of polyaniline directly on the surface of sawdust. the effects of some important parameters such as ph, initial concentration, sorbent dosage, exposure time and temperature o...

Synthetic CpG oligonucleotides are promising components of immunomodulatory drugs for the treatment and prophylaxis infectious diseases, cancers, allergies. Phosphorothioate modification stabilizes these compounds, contributing to achievement a clinical effect, but at same time changes their properties. We used diffusible fluorescent dye dihydroethidium non-diffusible 6-carboxy-2′,7′dihydrochlo...

In the field of adsorptive water remediation, Brunauer–Emmett–Teller (BET) equation is often used to describe sigmoid type II isotherm data. A standard a composite curve that comprises I and III at low high concentrations, respectively. The BET uses Langmuir-like function unique represent curve. However, ability this mathematical combination fit atypical isotherms somewhat limited. has been mod...

this study evaluates the effectiveness of adsorption process by granular activated carbon (gac) compared with a novel formulation of coagulation - flocculation process for dye removal from textile wastewater. in this regard, acidic, reactive, disperse and direct red dye are used to prepare the synthetic dye. dominant wave length for each dye is determined by spectrophotometeric method. using ga...

the clay crosslinked hydrogel nanocomposites were synthesized by graft copolymerization of acrylamide and itaconic acid in the presence of different amounts of laponite using ammonium persulfate and n,n,n',n'-tetramethylethylene diamine  )temed) as a free radical initiator and catalyst, respectively. the xrd patterns of nanocomposites showed that the clay layers are completely exfolia...

biosorption of acidic dyes using the live fern azolla filliculoides was studied in a discontinuous system. dye parameters, dye initial concentration and contact time were studied in temperature range of 25-30 ?c and ph=7. it was shown that increasing the 23initial concentration of dye and its contact time resulted in decreasing the dye taking quantity by the absorbent. also, type of dye has an ...

introduction: the powdered activated carbon treatment (pact) has been proposed as an alternative in wastewater treatment and wastewater biomass protection against toxic substances. this study aims to evaluate pact performance in treating dyes, acid orange, and remazol brilliant reactive blue. methods: this empirical- applied research was carried out in pilot scale in which different dye removal...

We have used the Doppler-free, perturbation-facilitated optical-optical double-resonance technique to investigate the vibrational, rotational, and hyperfine structure of the 3 (3)Pi double minimum state of NaK. Since this electronic state arises from an avoided crossing with the nearby 4 (3)Pi state, we observe striking patterns in the data that provide a sensitive probe of the electronic wave ...

A simple model is presented as an aid in understanding, first, the relative noise performance and, second, the noise reduction achievable by referencing, in different experimental approaches to single shot broadband coherent anti-Stokes Raman scattering (CARS). Qualitative agreement is obtained with previous experimental investigations of CARS noise. The broadband dye laser radiation is describ...