Pigments of natural origin have become a research trend, and fungi provide readily available alternative source. Moreover, developing novel processes that increase yields, reduce process time simplify downstream processing is increased interest. In this sense, work proposes an for Talaromyces atroroseus GH2 biomass re-utilization to produce pigments through consecutive batches using immobilized...

Transcription factor NF-kappaB is generally considered to be a heterodimer with two subunits, p50 and p65. The p50 subunit has been suggested to be generated from its precursor, p105, via the ubiquitin-proteasome pathway. During processing, the C-terminal portion of p105 is rapidly degraded whereas the N-terminal portion (p50) is left intact. We report here that a 23-amino-acid, glycine-rich re...

In speech perception, a functional hierarchy has been proposed by recent functional neuroimaging studies: core auditory areas on the dorsal plane of superior temporal gyrus (STG) are sensitive to basic acoustic characteristics, whereas downstream regions, specifically the left superior temporal sulcus (STS) and middle temporal gyrus (MTG) ventral to Heschl's gyrus (HG) are responsive to abstrac...

The hairpin loop structure and the downstream spacer element of histone mRNA precursors are both needed for efficient 3' end formation in vivo and in vitro. Though generally considered as a single processing signal, these two motifs are involved in different types of interaction with the processing machinery. Whereas RNA duplex formation between the downstream spacer element and the U7 small nu...

GeoBiotics, LLC and Kumba Resources (Pty) Ltd are jointly developing the GEOCOAT heap bioleaching technology and downstream processing technology for recovery of zinc from tailings at the Rosh Pinah Mine in Namibia. This paper outlines the development of a computer simulation for the bioleaching of sphalerite and the downstream processing. Computer modeling has shortened the product development...

Gene expression and processing during mouse male germ cell maturation (spermatogenesis) is highly specialized. Previous reports have suggested that there is a high incidence of alternative 30-processing in male germ cell mRNAs, including reduced usage of the canonical polyadenylation signal, AAUAAA. We used EST libraries generated from mouse testicular cells to identify 30-processing sites used...

2015 This article provides an overview of the current challenges and trends in bioprocessing, with a focus on recent advances in the affinity purification of the principal classes of biotherapeutics, including monoclonal antibodies, glycoproteins, vaccines and erythropoietin. Affinity chromatography is usually applied during large-scale protein purification; it involves affinity ligands of biol...

Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked polySia was developed using a disposable bioreactor for cultivation of Escherichia coli K1 and single-use membrane adsorbers for downstream processing (DSP). The cultivation...

In the past decade, it has been proven that cell therapy works in the lab, in preclinical settings, and in small-scale clinical trials. The need for large quantities of cells with high quality becomes crucial as product candidates advance into clinical trials and early commercial products. Throughout recent years, many companies had invested efforts in developing culturing technologies that wil...