BACKGROUND Methadone clinic-based directly observed antiretroviral therapy (DOT) has been shown to be more efficacious for improving adherence and suppressing human immunodeficiency virus (HIV) load than antiretroviral self-administration. We sought to determine whether the beneficial effects of DOT remain after DOT is discontinued. METHODS We conducted a post-trial cohort study of 65 HIV-inf...

Western blotting sprung from the need to develop a sensitive visual assay for the antigen specificity of monoclonal antibodies. The technique employed SDS-PAGE of protein antigens, electrophoretic replica transfer of gel-resolved proteins to unmodified nitrocellulose sheets, probing the immobilized antigens with hybridomas, and detection of antibody-antigen complexes with radiolabeled staphyloc...

brassica napus is an important oilseed crop and the yield loss due to fungal disease stem rot caused by sclerotinia sclerotiorum is a serious problem in cultivation of this crop. the pathogenesis-related (pr) protein, glucanase, hydrolyzes a major cell wall component, glucan, of the pathogenic fungi and acts as a plant defense barrier. in this study, a β-1,3-glucanase (bgn13.1) gene was isolate...