Introduction: Dynamic proteolysis, through the ubiquitin-proteasome system, has an important role in DNA transcription and cell cycle, is considered to modulate stress response synaptic plasticity. We investigated whether genetic variants ubiquitin carboxyl-terminal hydrolase 46 ( USP46 ) would be associated with post-traumatic disorder (PTSD) people exposure combat trauma using a case-control ...

Kisspeptin is a protein encoded by the KISS1 gene which behaves as key role stimulating gonadotropin releasing hormone (GnRH) neuron activity directly in reproductive axis. The objective of present study was to determine genetic diversity within intron 1 and verify their association with fecundity traits can be devoted marker assisted selection (MAS) for breeding selection. This established on ...

Background. Renal cell carcinoma (RCC) is asymptomatic up to severe stages and characterized by a high mortality rate, reaching 90 % with the development of metastatic process. Objective : determine group microRNA genes, methylation which associated progression disease, in particular, metastasis. Materials methods . Methylation-specific polymerase chain reaction representative sample RCC patien...

As experimental methods in molecular biology have rapidly progressed, the need for simple methodology to create RNA size markers has become increasingly apparent. Ribosomal RNAs are commonly used as size markers for larger RNAs in denaturing agarose gels (9); however, RNA markers are not commercially available for the characterization of <100 nucleotide (nt) RNAs by electrophoresis. This fact, ...

BACKGROUND Mycobacterium paratuberculosis is implicated as a possible cause of Crohn's disease. However, due to lack of an appropriate diagnostic method, this has been a subject of significant controversy. Our aim was therefore to develop a multiplex polymerase chain reaction (MPCR) for the detection of M. paratuberculosis DNA in Crohn's disease tissue. METHODS Biopsy samples were collected b...

Differential diagnosis of uterine leiomyomas and leiomyosarcomas is needed to determine whether the uterus can be retained. Therefore, biomarkers for uterine leiomyomas, and reliable and objective diagnostic methods have been desired besides the pathological diagnosis. In the present study, we identified 12 genes specific to uterine leiomyomas based on DNA methylation. Using these marker genes ...

Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of ...

Physical maps of rice chromosomes 4 and 7 were constructed by landing yeast artificial chromosomes (YACs) along our high-density molecular linkage map. Using 114 DNA markers, 258 individual YACs were located on chromosome 4. Sixty-two out of 258 YACs carried two or more DNA marker positions and formed 16 contigs which covered a total length of 17.1 cM. The other YACs were arranged to 23 positio...