Neq DNA polymerase is the first archaeal family B DNA polymerase reported to lack uracil recognition function and successfully utilize deaminated bases. We have focused on two amino acid residues (Y515, A523) in the fingers subdomain of Neq DNA polymerase, which were predicted to be located in the middle of the fingers subdomain, based on amino acid sequence alignment of the Neq DNA polymerase ...

Four T7 products, DNA polymerase, gene 4 protein, RNA polymerase, and DNA binding protein, have been purified from phage-infected cells. It has been previously shown (Hinkle, D. C., and Richardson, C. C. (1975) J. Biol. Chem 250, 5523-5529; Kolodner, R., and Richardson, C, C. (1978) J. Biol. Chem 253, 574-584) that two T7 products, DNA polymerase and gene 4 protein, catalyze extensive synthesis...

DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...

DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...

DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...

DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...

An RNA-dependent DNA polymerase was isolated from purified virions of endogenous oncornaviruses released by the MOPC-315 murine myeloma cell line. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified enzyme was found to consist of two major polypeptides with molecular weights of about 28,000 and 26,500. The active enzyme had a molecular weight of approximately 56,000, as...

The DNA polymerase gene from the archaeon Pyrococcus sp. strain KOD1 (KOD DNA polymerase) contains a long open reading frame of 5,013 bases that encodes 1,671 amino acid residues (GenBank accession no. D29671). Similarity analysis revealed that the DNA polymerase contained a putative 3'-5' exonuclease activity and two in-frame intervening sequences of 1,080 bp (360 amino acids; KOD pol intein-1...

Changes in DNA polymerase alpha activity accompanying tissue development have been well established in several systems. In most cases, DNA polymerase alpha activity decreases with development. Here, we report observed changes in DNA polymerase alpha activity throughout embryonic chicken brain (ECB) development. The level of DNA polymerase alpha activity was found to gradually decrease by 60% (2...

Replication of hepadnaviruses involves reverse transcription of an intermediate RNA molecule. It is generally accepted that this replication scheme is carried out by a virally encoded, multifunctional polymerase which has DNA-dependent DNA polymerase, reverse transcriptase, and RNase H activities. Biochemical studies of the polymerase protein(s) have been limited by the inability to purify usef...