Streamline and Improve Your Genotyping and Genetic Studies Applied Biosystems has designed BloodPrepTM Chemistry for quick and efficient DNA purification from many sample matrices, including: fresh and frozen whole blood from various animal species in all common anticoagulants, isolated blood cells such as “buffy coat” or leukocyte fractions, cultured cells, and buccal swabs. Novel reagent form...

Cell-free DNA is of increasing interest in different fields, such as cancer research, prenatal diagnosis, and the diagnosis of nonmalignant diseases. The quantity of free-circulating DNA in plasma, serum, and other body fluids is usually low and its isolation is still a challenge. Here we evaluate the application of the new commercially available NucleoSpin Plasma XS Kit for the isolation of ce...

A standardised commercially available radioimmunoassay kit for the detection of antibodies to native DNA (N-DNA) has been evaluated in clinical practice. This test system is shown to be a reliably reproducible method of detecting these antibodies. In addition, evaluation of the purity of the radiolabelled test antigen in this assay has shown it to be almost entirely double stranded (native) DNA...

AIM Discussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues. METHODS DNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chlor...

There continues to be a focus on the development of recombinant human monoclonal antibodies (rhuMAb IgGs) for therapeutic use, with dozens reaching the market in the last decade. Therapeutic proteins of the scale needed for treatment of even a small population require industrial scale production using a variety of bioprocessing methods including recombinant cell line expression systems, chromat...

background: soybean oil is one of the highly consumed vegetable oil worldwide. nowadays, usage of genetically modified (gm) soybean seeds for soybean oil production is constantly increasing. the recommended methods for gmo detection are based on analysis of residual dna in vegetable oil and highly processed food. however, the successful amplification of isolated dna depends on the efficiency of...

Oligonucleotides are synthesized and purified in a single column filled with a mixture of nucleoside-loaded and underivatized, high-cross link, non-swelling polystryene beads. A new oligonucleotide production system has been developed to completely automate-the entire process of sequence entry, phosphoramidite synthesis, cleavage, deprotection, purification, quantitation, and sample collection....

muslims nowadays need to be present on the international stage. this involves living together with non-muslims, especially the people of the book. this sort of life and interaction has different political, cultural, economic, and military aspects. the belief that non-muslims are impure (najes) causes hardship to the muslims; and is incompatible with human dignity, which is acknowledged by the q...

Brix and apparent purity are well known to be biased estimators of dry solids and true purity respectively, particularly on lower purity streams. Correlations which estimate dry solids from brix and apparent purity and estimate true purity also from brix and apparent purity are presented. The suitability of these correlations is evaluated in the light of available historical data.