Nucleoid-associated proteins (NAPs) are chromosome-organizing factors, which affect the transcriptional landscape of a bacterial cell. HU is an NAP, which binds to DNA with a broad specificity while homologous IHF (Integration Host Factor), binds DNA with moderately higher specificity. Specificity and differential binding affinity of HU/IHF proteins towards their target binding sites play a cru...

Abstract The coronavirus disease 2019 (COVID‐19) pandemic has devastated families and disrupted healthcare, economies societies across the globe. Molecular recognition agents that are specific for distinct viral proteins critical components rapid diagnostics targeted therapeutics. In this work, we demonstrate selection of novel DNA aptamers bind to SARS‐CoV‐2 spike glycoprotein with high specif...

Among the most important classes of regulatory proteins are the sequence-specific DNA-binding proteins that control transcription through the occupancy of discrete DNA sequences within genomes. Currently, this class of proteins encompasses at least 37 distinct structural superfamilies and more than 100 distinct structural motifs. In this paper, we examine the transcriptional regulator Wor1, a m...

The cold-shock domain has a deceptively simple architecture but supports complex biology. It is conserved from bacteria to man and representatives in all kingdoms of life. Bacterial proteins consist single some, not are induced by cold shock. Cold-shock domains human often associated with natively unfolded protein segments more rarely other folded domains. share five-stranded all-antiparallel ?...

The E2A gene encodes two alternatively spliced products, E12 and E47. The two proteins differ in their basic helix-loop-helix motifs (bHLH), responsible for DNA binding and dimerization. Although both E12 and E47 can bind to DNA as heterodimers with tissue-specific bHLH proteins, E12 binds to DNA poorly as homodimers. An inhibitory domain in E12 has previously been found to prevent E12 homodime...