The aim of this work is to detect acetamiprid using electrochemical capacitance spectroscopy, which widely used as a pesticide in agriculture and harmful humans. We have designed aptasensing platform based on the adsorption DNA aptamer lipoic acid-modified MoS2 nano-sheets. biosensor takes advantage high affinity single-stranded sequences stability nano-sheets assured by covalent attachment aci...

Background. Aptamers are small single-stranded DNA or RNA molecules that have an affinity for a specific target molecule. The main method of aptamers construction is the technology systematic evolution ligands with exponential enrichment (SELEX). However, exact approach depends on nature molecules, and selected optimized by each researcher independently. article describes technique production t...

Abstract Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) as input signal fluorescence output signal, if DNA (DNA-L), (DNA-S), ATP were present at the same time, on...

Functional nucleic acid (FNA)-based sensing systems have been developed for efficient detection of a wide range of biorelated analytes by employing DNAzymes or aptamers as recognition units. However, their intracellular delivery has always been a concern, mainly in delivery efficiency, kinetics, and the amount of delivered FNAs. Here we report a DNA dendrimer scaffold as an efficient nanocarrie...

Human noroviruses (HuNoV) are the leading cause of acute viral gastroenteritis and an important cause of foodborne disease. Despite their public health significance, routine detection of HuNoV in community settings, or food and environmental samples, is limited, and there is a need to develop alternative HuNoV diagnostic reagents to complement existing ones. The purpose of this study was to sel...

BACKGROUND Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). More efficient methods of controlling and detecting STIV infections are urgently needed.  METHODS In this study, we generated eight single-stranded DNA (ssDNA) aptamers against STIV using systematic evolution of ligands by exponential enrichment (SELEX). RESULTS...

New natural and chemically modified DNA aptamers that inhibit HIV-1 activity at submicromolar concentrations (presumably via preventing viral entry into target cells) are reported. The new DNA aptamers were developed based on known intramolecular G-quadruplexes (G4s) that were functionally unrelated to HIV inhibition [the thrombin-binding aptamer and the fragment of the human oncogene promoter ...

We describe herein the development of a high affinity and specific DNA aptamer as a new ligand for use in liposomal nanoparticles to target cultured mouse tumor endothelial cells (mTECs). Active targeted nanotechnology based drug delivery systems are currently of great interest, due to their potential for reducing side effects and facilitating the delivery of cytotoxic drugs or genes in a site ...

Aptamer-based fluorescence anisotropy (FA) assay combines the advantages of affinity aptamers in good stability, easy generation, and facile labeling and the benefits of FA in homogeneous analysis, such as robustness, simplicity, and high reproducibility. By using a fluorophore-labeled aptamer, FA detection of a small molecule is not as easy as detection of protein because the binding of a smal...

Lupus nephritis (LN) occurs in up to 60% of patients with systemic lupus erythematosus (SLE). Despite current development immunosuppressant agents, LN still impairs the survival and quality life SLE patients. Receptor for advanced glycation endproducts (RAGE) is a multi-ligand receptor that belongs immunoglobulin superfamily, which strongly associated innate immune system. In present study, we ...