Ubiquitination, a highly versatile process of protein homeostasis, participates in the degradation vast majority cellular proteins. In addition to proteolytic function, ubiquitination can also conduce modulate processes independent proteolysis, such as signal transduction, trafficking, DNA repair and so on. Apoptosis modulates cell quantity via orderly removing damaged cells effectively plays f...

Background: Cyclophosphamide is widely prescribed as an anti-cancer drug and used immunosuppressant. Hemorrhagic cystitis one of the common complications cyclophosphamide intake. We hypothesized that endoplasmic reticulum stress-related proteins could be altered in urothelium treated with cyclophosphamide. Objectives: checked effect on expression various Vero cells. Methods: cells varying doses...

We performed a detailed cancer VS normal analysis to explore the expression and prognostic value of minichromosome maintenance (MCM) proteinsin human sarcoma. The mRNA levels MCM family genes in sarcoma were analyzed using data from ONCOMINE, GEPIA CCLE databases. KEGG database was used analyze function MCM2–7 complex DNA replication cell cycle. QRT-PCR western blot confirm differential key MCM...

Glioblastoma (GBM) is an aggressive malignancy with a high rate of tumor recurrence after treatment conventional therapies. Parthenolide (PTL), sesquiterpene lactone extracted from the herb Tanacetum parthenium or feverfew, possesses anticancer properties against wide variety solid tumors. In present study, series PTL derivatives were synthesized and screened. An inhibitor, dimethylaminoparthen...

The role of the N-terminal cleavage product of poly(ADP-ribose) polymerase (PARP) on UV mediated apoptosis was investigated in cultured HeLa cells. Ultrastructural analysis of cells expressing caspase-resistant PARP (PARP(D214A)) revealed the typical features of necrosis following UV treatment. However, cells co-expressing PARP(D214A) with the N-terminal fragment of PARP containing the DNA-bind...

Complex multicellular organisms have evolved mechanisms to ensure that individual cells follow their proper developmental and somatic programs. Tumorigenesis, or uncontrolled cellular proliferation, is caused by somatic mutations to those genetic constraints that normally operate within a tissue. Genes involved in DNA repair and apoptosis are particularly instrumental in safeguarding cells agai...

Introduction Extensive DNA damage and inefficient DNA repair might be responsible for some of the pathogenic features in patients suffering from asthma. To determine whether DNA adducts can be used as a “dosimeter” for asthma disease severity, we measured the DNA adducts level in lung of mouse with house dust mite (HDM)induced allergic airway inflammation, as the disease progresses. Apoptosis o...

Cancer is the second leading cause of death worldwide. Cisplatin has challenged cancer treatment; however, resistance and side effects hamper its use. New agents displaying improved activity more reduced relative to cisplatin are needed. In this work we present synthesis, characterization biological activities three complexes with quinoline-substituted 2,2′:6′,2″-terpyridine ligand: [Pt(4′-(2-q...

BACKGROUND The aim of this study was to examine the role of apoptosis and reactive oxygen species (ROS) in inducing DNA damage in ejaculated spermatozoa. METHODS We examined ejaculated spermatozoa from 31 patients examined for infertility and 19 healthy donors for apoptosis, production of ROS and DNA damage using annexin V binding, chemiluminescence assay and sperm chromatin structure assay. ...

To clarify whether lipids deposited in human atheromatous lesions induce apoptosis of vascular smooth muscle cells (SMC) and to identify the main component in deposited lipids responsible for inducing apoptosis, we examined the effect of lipids extracted from human atheromatous lesions on apoptosis of cultured SMC and analyzed the content of cholesterol in the lipids. When lipids extracted from...