1 Abstract The general objective of the proposed research is to use ab initio techniques to develop a fundamental understanding of the relationship between the atomic and electronic structure of nanostructured materials and their functional properties. Our research will both expand upon the work performed in the past year using XSEDE resources and embark on the study of important new systems. I...

The recent demonstration of genomic imprinting of DLK1 and MEG3 on human chromosome 14q32 indicates that these genes might contribute to the discordant phenotypes associated with uniparental disomy (UPD) of chromosome 14. Regulation of imprinted expression of DLK1 and MEG3 involves a differentially methylated region (DMR) that encompasses the MEG3 promoter. We exploited the normal differential ...

Oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. Our study compared the action of a commercial laccase from Aspergillus oryzae and a rich extract from Pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: Drimaren Blue X-3LR (DMBLR), Drimaren Blue X-BLN (DMBB...

We present subclone sensitive cell phenotypic pharmacology of ligands at the β2-adrenergic receptor (β2-AR) stably expressed in HEK-293 cells. The parental cell line was transfected with green fluorescent protein (GFP)-tagged β2-AR. Four stable subclones were established and used to profile a library of sixty-nine AR ligands. Dynamic mass redistribution (DMR) profiling resulted in a pharmacolog...

Polycomb Repressive Complex 2 (PRC2) function and DNA methylation (DNAme) are typically correlated with gene repression. Here, we show that PRC2 is required to maintain expression of maternal microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) from the Gtl2-Rian-Mirg locus, which is essential for full pluripotency of iPSCs. In the absence of PRC2, the entire locus becomes transcriptionally re...

The Sry (sex determining region on Y chromosome) gene is a master gene for sex determination. We previously reported that the Sry gene has tissue-dependent and differentially methylated regions (T-DMRs) by analyzing the DNA methylation states at CpG sites in the promoter regions. In this study, we found unique non-CpG methylation at the internal cytosine in the 5'-CCTGG-3' pentanucleotide seque...

Abstract Objectives This study examines the sensitivity of development dorsal metacarpal ridge (DMR) to frequency knuckle‐walking during ontogeny in chimpanzees, and compares prevalence prominence DMR hominoids baboons. Materials Methods We characterized type quadrupedalism wild chimpanzees different ages from Ngogo community, Uganda. Using museum specimens, we quantified third angle height Pan...

several processes have been proposed for natural gas liquefaction due to the vast utilization of lng as a reliable and relatively easy to use fuel. even though the merits and demerits of different process have been studied, a dearth of comprehensive technical and economical comparative investigation of these methods makes further broad examination a necessity. this article is presented to addre...