This study investigated the effects and mechanisms of miR-132 related to permeability mobility human retinal pigment epithelium ARPE-19 cells in high-glucose (HG) condition. were cultured normal HG condition identified by immunofluorescence staining. Cell viability was assessed MTT assay, cell FITC-dextran assay wound healing assay. Different miRNA mRNA expression levels determined quantitative...

Nasopharyngeal culture, direct immunofluorescence, and serology of acute-phase and paired serum specimens were compared for the laboratory diagnosis of infections due to Bordetella pertussis in a community-based pediatric population with both high vaccine usage and high pertussis incidence. In 77 (37%) of 210 patients evaluated, one or more tests were positive for pertussis. A clinical illness ...

background and aims: respiratory syncytial virus (rsv) infection is common in infants and young children. in infants younger than one year old it may cause bronchiolitis and pneumonia which requires hospitalization. accurate and rapid diagnosis of the disease will help proper treatment of the disease and prevents further complications. methods: specimen taken from respiratory tract of sick chil...

DH: dermatitis herpetiformis DIF: direct immunofluorescence INTRODUCTION Dermatitis herpetiformis (DH) is an autoimmune, subepidermal blistering disease associated with gluten sensitivity. It commonly manifests as intensely pruritic papules and vesicles on the extensor surfaces of extremities in a symmetric distribution. Classic histologic findings and data from direct immunofluorescent (DIF) s...

BACKGROUND The pathogenesis of ANCA-associated pauci-immune glomerulonephritis has not been fully elucidated. Several studies had suggested that complement deposition could be detected in renal histopathology. The current study investigated the clinical and pathological significance of complement deposition in renal histopathology of patients with ANCA-associated pauci-immune glomerulonephritis...

anTiBOdies An antibody is a protein complex produced by B cells that initiates an immune response against a target antigen. The basic organization of an antibody includes two functional domains that, together, resemble the letter Y (Figure 1, left). The Fab (fragment having the antigen binding site) domain makes up the arms of the Y, and at the end of each arm is a variable region responsible f...