CPS-1 is a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which ov...

Identification of Pseudomonas aeruginosa Clinical Strains Harboring the blaVIM-2 Metallo-β-Lactamase Gene in Akita Prefecture, Japan Jun Yatsuyanagi*, Shioko Saito, Yuko Ito, Kazuko Ohta, Jun Kato, Seizaburo Harata, Noriyuki Suzuki and Ken-ichi Amano Akita Prefectural Institute of Public Health, Akita 010-0874, Akita Kumiai General Hospital, Akita 011-0911, Odate Municipal Hospital, Oodate 027-...

background :   ampc beta lactamases play a significant role in creating resistance to third generation cephalosporins worldwide. they mostly express on chromosome of enterobacteriaceae especially escherichia coli and cause consequential problem in clinical treatment and lead to failure in diagnosis and phenotypic test recommended by clinical and laboratory standards institute.   methods : total...

Background: Extended-spectrum β-lactamase (ESβL) or metallo-β-lactamase (MβL) production by gram-negative bacteria in immunocompromised patients poses a serious therapeutic challenge for infection control and is associated with infections higher morbidity/mortality, especially developing countries. This study aimed to phenotypically evaluate the of ESβL as well MβL 75 bacterial isolates from cl...

The objective of this study was to evaluate the susceptibility of metallo-β-lactamase (MBL)-producing Acinetobacter baumannii (A. baumannii) clinical isolates to biocides. We also determined the prevalence and correlation of efflux pump genes, class 1 integron and MBL encoding genes. In addition, blaVIM, blaNDM-1, qacE and qacEΔ1 nucleotide sequence analysis was performed and compared to sequen...

Carbapenems are one of the last lines of defense for Gram-negative pathogens, such as members of the Enterobacteriaceae. Despite the fact that most carbapenems are resistant to extended-spectrum β-lactamase (ESBL), emerging metallo-β-lactamases (MBLs), including New Delhi metallo-β-lactamase 1 (NDM-1), that can hydrolyze carbapenems have become prevalent and are frequently associated with the s...