The demand for energy has been promptly increasing due to rapid growing world population and increased growth of industries. In the pursuit of renewable energy production, the anaerobic digestion of organic wastes was a proved technology that is shaping up to be the one of the reliable source of alternate energy. Even though the anaerobic digestion is an effective method of renewable energy pro...

Several proteomics approaches are available that are defined by the level (protein or peptide) at which analysis takes place. The most widely applied method still is bottom-up proteomics where the protein is digested into peptides that can be efficiently analyzed with a wide range of LC-MS or MALDI-TOF-MS instruments. Sample preparation for bottom-up proteomics experiments requires several trea...

Effects of different pretreatment methods on sludge dewaterability and polycyclic aromatic hydrocarbons (PAHs) degradation during petrochemical sludge anaerobic digestion were studied. Results showed that the total biogas production volume in the thermal pretreatment system was 4 and 5 times higher than that in the ultrasound pretreatment and in the control system, and the corresponding volatil...

Sequential fractionation has helped improving our understanding of the lability and bioavailability of P in soil. Nevertheless, there have been no reports on how manipulation of the different fractions prior to analyses affects the total P (TP) concentrations measured. This study investigated the effects of sample digestion, filtration, and acidification on the TP concentrations determined by I...

The relationship between the nature of in vitro digestion products and protein quality evaluation in rats was established. Eleven protein sources of animal and vegetable origin and of various purities were digested by pepsin then by pancreatin in a dialysis bag of 1,000 molecular weight cutoff. Animal proteins were generally better digested in vitro than vegetable proteins. Amino acid release p...

Anaerobic digestion is an attractive method for waste stabilization with the potential generation of biogas and stabilized waste residue. Development of a treatment process at short digestion period with maximum process efficiency is the objective of this research. This paper presents the optimization performance between the combined anaerobic digestion and sequential staging concept in thermop...

A monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activation and trypsin modification. The choice of acrylamide as co-monomer was foun...

Anaerobic digestion of animal manure in biogas digesters has shown promise as a technology in reducing the microbial load to safe and recommended levels. We sought to treat dairy manure obtained from the Fort Hare Dairy Farm by investigating the survival rates of bacterial pathogens, through a total viable plate count method, before, during and after mesophilic anaerobic digestion. Different mi...