Measurements of two-photon-excited fluorescence (TPF) of fluorescein and Rhodamine 6G in various solvents were performed with a continuous-wave (cw) laser for excitation and an acousto-optic tunable filter for spectral dispersion. Interestingly, the cw laser excitation produced an unwanted thermal-lens effect when the measurements were performed in solvents that absorb the excitation laser ligh...

Developing green solvents with low toxicity and cost is an important issue for the biochemical industry. Synthetic ionic liquids and deep eutectic solvents have received considerable attention due to their negligible volatility at room temperature, high solubilization ability, and tunable selectivity. However, the potential toxicity of the synthetic ionic liquids and the solid state at room tem...

Background: D-Phenylglycine aminotransferase (D-PhgAT) is highly beneficial in pharmaceutical biotechnology. Like many other enzymes, D-PhgAT suffers from low stability under harsh processing conditions, poor solubility of substrate, products and occasional microbial contamination. Incorporation of miscible organic solvents into the enzyme’s reaction is considered as a solution...