Heparan sulphate proteoglycan is widely distributed, being found inserted into plasma membranes of most cells (Kjellenetal., 1981 ; Norlingetal., 1981)and asa significant component of basement membranes (Kanwar et al., 1980; Hassel et al., 1980). As a strong polyanion, heparan sulphate interacts with many components under physiological conditions, the specificity of these interactions depending...

The structure of dermatan [35S]sulphate-chondroitin [35S]sulphate copolymers synthesized and secreted by fibroblasts in culture was studied. 35S-labelled glycosaminoglycans were isolated from the medium, a trypsin digest of the cells and the cell residue after 72h of 35SO42-incorporation. The galactosaminoglycan component (dermatan sulphatechondroitin sulphate copolymers) was isolated and subje...

Newly synthesized rat glomerular [35S]proteoglycans were labelled in vivo after injecting Na2[35S]SO4 intraperitoneally. At the end of the labelling period (7 h) the kidneys were perfused in situ with 0.01% (w/v) cetylpyridinium chloride. This fixed proteoglycans in the tissue and increased their recovery 2-3-fold during subsequent isolation of glomeruli from the renal cortex. The glomeruli wer...

1. Expression of the recombinant human excitatory amino aid transporters, EAAT1 and EAAT2, in Xenopus laevis oocytes allows electrogenic transport to be studied under voltage clamp conditions. 2. We have investigated the transport of the pharmacological substrate, L-serine-O-sulphate transport by EAAT1 and EAAT2. The EC50 values for L-serine-O-sulphate transport by EAAT2 showed a steep voltage-...

Magnesium sulphate has been used in the acute treatment of migraines; some studies found it to be a highly effective medication in the acute control of migraine pain and associated symptoms. This randomized, double-blind, placebo-controlled study assesses the effect of magnesium sulphate on the pain and associated symptoms in patients with migraine without aura and migraine with aura. Sixty pat...

1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and...

The Oxford Whole Body Counter was used to measure absorption from various therapeutic preparations of iron in five groups of subjects. Succinic acid enhanced absorption of iron when added to a solution of ferrous fumarate, but not when given with tablets of ferrous fumarate or ferrous sulphate. Ferrous fumarate plus ascorbic acid was absorbed better than ferrous fumarate alone but no better tha...

Controversial views exist with regards to the use of intravenous magnesium sulphate in patient with severe asthmatic attack. The benefit of intravenous magnesium sulphate may be masked by the pooling of patients with different pathophysiology. The subsets of patients with severe asthmatic attack who have not responded to nebulised bronchodilator therapy and intravenous steroid therapy may benef...

Sulphate-reducing bacteria (SRB) are those prokaryotic microorganisms, both bacteria and archaea, that can use sulphate as the terminal electron acceptor in their energy metabolism, i.e. that are capable of dissimilatory sulphate reduction. Most of the SRB described to date belong to one of the four following phylogenetic lineages (with some examples of genera): (i) the mesophilic d-proteobacte...