1. Computer averaging of multiple scans was used to refine the circular dichroism spectrum of bovine liver glutamate dehydrogenase, revealing well-defined structure in the aromatic region. 2. The circular dichroism ofNAD+ bound to glutamate dehydrogenase is strongly negative at 260nm, probably owing to immobilization of the adenosine moiety. Loss of the characteristic adenine-nicotinamide inter...

Circular dichroism and difference ultraviolet visible spectra were obtained for cobalt hemoglobin derivatives. At 287 nm the ellipticity difference between the oxy- and deoxycobaltohemoglobin is about one-half as great as that for the native proteins indicating smaller quaternary conformational changes for the former. Deoxygenation increases the Soret rotational strengths of both iron and cobal...

We study valley and spin dynamics in monolayer molybdenum diselenide by polarization-resolved femtosecond transient absorption spectroscopy. Valley- and spin-polarized excitons are injected by a circularly polarized laser pulse, with an excess energy of 120 meV. Relaxation of the valley polarization is time-resolved by measuring dynamical circular dichroism of a linearly polarized probe pulse t...

The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) in a pH-dependent manner, and this fluorescence inactivation could be partially...

15-Crown-5-appended metalloporphyrin causes a K(+)-driven self-organization to bind a bifunctional guest ditopically, thereby allowing the circular dichroism (CD) detection of chirality induced in the ensemble when chiral amines are employed as the guest; the chiroptical properties are discussed.

This chapter presents the process of interaction of light, mainly in the xray range, with matter. The subject is introduced by some experimental evidences of x-ray absorption, emission or scattering by different kinds of material. The ways of describing the electromagnetic wave field are then briefly presented before developing its interaction with an electron in an atom. This process is then a...

The single histidine residue (His-15) in hen egg white lysozyme (EC 3.2.1.17) was chemically modified by diethyl pyrocarbonate (DEPC) to form exclusively the mono-N-carbethoxyimidazole adduct (second order rate constant of 252 +/- 16 M-1 min-1). Irreversible biscarbethoxylation of the His-15 imidazole ring by DEPC was observed when lysozyme was pretreated with 2-mercaptoethanol (2-ME), 2-ME plu...

We report transient electric dichroism studies on monodisperse rod-like DNA molecules. By using restriction fragments and DNAs of known length, it is shown that the orientation time is accurately predicted by the theoretically calculated rotational diffusion coefficient. The field dependence of the steady-state dichroism values is not consistent with the induced electric dipole orientation mech...

We have observed the magnetic-phase transitions of CeSb and CeBi by a magnetic circular dichroism spectroscopy in the infrared region. The phase boundary is similar to that detected by neutron scattering. The optical spectra re#ect the electronic structures in the ordered states. The electronic structure and also the origin of the magnetic transition are discussed. ( 2000 Elsevier Science B.V. ...