The study of the metabolism of 2-aminofluorene (AF) has become of considerable importance, since it has been demonstrated that 2-acetylaminofluorene (AAF) is rapidly deacetylated in the rat (8, 13). This observation indicates that AF is a major product in the degradation of AAF by the rat and that the metabolism of AAF may be iden tical with that of AF. The metabolism of AF is also of interest ...

After the intravenous injection of unconjugated [(3)H]bilirubin into normal Sprague-Dawley and Wistar R rats, radiolabeled bile pigments rapidly accumulated in the liver. By 1.5 min after injection, an average of 36% of the injected isotope was present in liver homogenates. Between 3 and 15 min, 37-64% of the total intrahepatic radiolabeled bilirubin was conjugated, as demonstrated by extractio...

Intensely blue dye-azoproteins have been prepared by diazotization and coupling of the highly indiffusible blue dye T-1824, Evans blue, with various serum proteins and egg albumin. The products, whether purified by precipitation with alcohol or by chromatography, have a constant dye-to-protein ratio and tests have shown them to be essentially free from unlinked dye. An extremely diffusible dye,...

a method for the simultaneous determination of sulfamethoxazole (smx) and trimethoprim (tmp), based on a direct determination of smx after diazotization and coupling with 2-naphthol by visible spectrophotometry and an indirect determination of tmp in the uv region by difference was developed. by the suggested procedure, it was possible to analyze smx and tmp in pharmaceutical preparations witho...

a simple and rapid spectrophotometric method for the determination of nitrite in water and soil preparation samples has been developed. determination of nitrite is based on diazotization of p-nitro aniline with nitrite and subsequent coupling with 4-amino-3-hydroxynaphthalene-1-sulfonic acid in hydrochloric acid. the factors affecting the reaction i.e. concentration of p-nitro aniline, concentr...

The syntheses and properties of 8-aza-7-deazapurine (pyrazolo[3,4-d]pyrimidine) ribonucleosides related to 2-aminoadenosine and isoguanosine are described. Glycosylation of 8-aza-7-deazapurine-2,6-diamine 5 with 1-O-acetyl-2,3,5-tri-O-benzoyl-beta-D-ribofuranose (12) in the presence of BF(3) x Et(2)O as a catalyst gave the N(8) isomer 14 (73%) with a trace amount of the N(9) isomer 13a (4.8%). ...

The German organic chemist Johann Peter Griess (1829–88), who first developed the diazotization of aryl amines (the key reaction in the synthesis of the azo dyes), and a major figure in the formation of the modern dye industry, worked for more than a quarter of a century at the brewery of Samuel Allsopp and Sons in Burton upon Trent, which, owing to the presence of several notable figures and a...

When metronidazole (Flagyl) is prescribed for trichomoniasis or lambliasis, it is unusual for the treatment not to be followed-up; however, it is possible that the patient does not take the drug, and the doctor may wonder whether the drug is being properly absorbed. It is also thought that metronidazole decreases the desire for alcohol in alcoholics. Here the co-operation of the patient may be ...

The study of the metabolism of 2-aminofluorene (AF) has become of considerable importance, since it has been demonstrated that 2-acetylaminofluorene (AAF) is rapidly deacetylated in the rat (8, 13). This observation indicates that AF is a major product in the degradation of AAF by the rat and that the metabolism of AAF may be iden tical with that of AF. The metabolism of AF is also of interest ...